Attractin蛋白在大鼠睾丸和附睾中的免疫组织化学定位

目的 :探讨Attractin蛋白在成熟大鼠睾丸和附睾组织中的分布。　方法 :健康成年雄性SD大鼠 2 0只 ,灌流取睾丸和附睾组织固定 ,石蜡包埋和冰冻切片。用免疫组化法和间接免疫荧光方法检测Attractin蛋白在成熟大鼠睾丸和附睾组织中的表达。　结果 :睾丸组织间质细胞、睾丸精曲小管管周肌样细胞和各级生精细胞 (精原细胞、初级精母细胞和精子细胞 )、支持细胞呈阳性反应 ,主要表达于胞膜及胞质。睾丸间质细胞表达略强于生精细胞。附睾头、体、尾均未见表达。　结论 :Attractin蛋白在成熟大鼠睾丸组织间质细胞和生精细胞中有较强的表达 ,其生理功能尚有待进一步探讨。

Immunohistochemical Localization of Attractin in the Testis and Epididymis of Mature Male Rats

Objective: To localize the attractin protein in the testis and epididymis of mature male rats. Methods: Testes and epididymides were obtained from mature male Sprague dawley rats( n =20). Tissues were fixed and prepared for immunohistochemical(IHC) and indirect immunofluorescent(IIF) assay,carried out with antiserum against rat attractin. Results: In the testis of the male rat,there was distinct immunopositive staining on cell membrane and cytoplasm within Leydig cells,primitive spermatogonia,primary spermatocytes,spermatids,Sertoli cells,and peritubular myoid cells. In the epididymis,including caput,corpus,cauda,there was no definitive immunopositive staining within the efferent ductule and epididymal duct. Conclusion: Attractin is expressed in the male rat reproductive system and localized within Leydig cells and germ cells. It may be invoved in acting on the reproductive system. And its physiological function has yet to be further studied.