p16蛋白在成釉细胞瘤中的表达

目的：研究成釉细胞瘤（ameloblastoma,AB)星网状层细胞增殖与分化的特点，探讨其生物学意义。方法：应用p16蛋白单克隆抗体免疫组化S－P法检测40例AB，其中原发17例，复发16例，恶变7例，此外用口腔粘膜鳞癌4例，淋巴结转移癌2例，基底细胞癌7例作对照观察。结果：40例AB的p16蛋白的缺失率为52．5％，其中，原发AB29．4％，复发AB62．5％，恶变AB85．7％，3组间P＜0．05。p16蛋白在多已发生角化的上皮样细胞当中有弱表达，但在星网支中原始胚样细胞多表达为阴性。结论：AB的发生与p16蛋白的缺失有关。随着复发与恶变的变化，p16蛋白缺失率增加（P＜0．05）。具有显著性意义。p16蛋白的阳性表达与细胞的分化状态有关。

Expression of p16 in Ameloblastoma

Objective: Our aims were to study the expression of p16 protein in ameloblastoma (AB) and to investigate the proliferative and differention characteristics of stellate reticulum in AB. Methods: The immunoactivity of p16 protein in 40 cases of AB (17 cases of primitive AB, 16 of recurrent AB , 7 of malignant AB ) was determined by using Streptavidin-Biotin method. Seven cases of basal cell carcinomas, 4 of squamous cell carcinomas, 2 of carcinomas with lymphatic metastasis were as positive control. Results: The ratios of expression lost of p16 protein in primitive AB, recurrent AB, and malignant AB were 29.4%, 62.5%, 85.7% respectively ( P <0.05). The p16 protein expressed slightly in the squamous epithelium cell but negatively in embryonoid cell in stellate reticulum in AB. Conclusion: Development of AB is related with the lost of p16 protein. A great difference in expression level of p16 protein existed between recurrent, malignant, and primitive AB. Expression of p16 in AB is related with cell differention.