不同细胞因子基因转导对小鼠B16细胞体内生长及免疫原性的影响

目的：通过比较不同细胞因子基因转导对肿瘤细胞体内生长及对机体免疫活性的影响，探讨基遥作用机制及因子间的协同效果。方法：采用逆转录病毒介导的方法将ＩＬ－６／ＩＬ－２融合基因及ＩＬ－６、ＩＬ－２单基因分别导入小鼠Ｂ１６黑色素瘤细胞中，检测基因修饰瘤细胞体内成瘤及对机体ＬＡＫ、ＣＴＬ活性的诱导。结果：三种基因修饰的肿瘤细胞均可表现出体内生长抑制及免疫原性增强，但融合基因转导细胞的改变，较单一基因修饰细胞

Effect of various cytokine gene transduction on B16 cell growth in vivo and immunogenicity

Objective:To investigate the modulation mechanism of various cytokine gene transfer on tumor cell biological characteristics and the synergetic effect between cytokines,studied the effect of single IL 2,IL 6 gene or IL 6/IL 2 fusion gene transduction on tumor cell growth in vivo and immunogenicity.Methods:Constructed three retroviral vectors contained hIL 6,hIL 2 single gene or hIL 6 /IL 2 fused gene.The vectors were transferred into B16 melanoma cell line respectively and the influence of gene transfer on tumorigenicity and the LAK and CTL cytotoxic activities of vaccinated mice splenocytes were examined.Results:B16 cells modified by various gene showed decreased tumorigenicity and enhanced LAK,CTL cytotoxicity.The changes of fusion gene transfectant is more obvious than that of single gene transfectants.Conclusion:The efficiency of tumor cell modification induced by various cytokine gene transfer is different,as the synergetic effect ,the effect induced by IL 6/IL 2 fusion gene transfer is superior to that of the IL 6 and IL 2 gene alone.