Effects of procaine on intracellular pH and its regulation: measurements with pH-selective micro-electrodes in Retzius neurones of the leech |
| |
Authors: | H Moser D Heinemeyer M Asal W R Schlue |
| |
Institution: | (1) Institut für Zoologie I, Universität Düsseldorf, D-4000 Düsseldorf, Federal Republic of Germany;(2) Institut für Zoologie, Technnikerstrasse 25, A-6020 Innsbruck, Austria |
| |
Abstract: | The experiments were performed with double-barelled micro-electrodes in Retzius neurones of the leech in order to see if the tertiary amine local anesthetic procaine affects intracellular pH (pHi) and its regulation. Lasting about 20 min, exposures to procaine (3 mM) saline caused an intracellular alkalosis of about 0.2 pH units which gradually degradated; its removal initiated a fast decrease of pHi resulting in an acidosis, from which the cells recovered. Detailed studies, involving CO2 or NH
4
+
acid loaded cells as well as inhibition of the pHi regulating exchange mechanism by various drugs or exposures to Na-free salines suggest, that neither the Na+–H+, nor a probably Na+-dependent HCO
3
–
–Cl– exchange are affected by procaine. We propose a model for procaine action on pHi assuming that neutral procaine passes the membrane easily through lipophilic pathways. The degradation of alkalinity during procaine exposure hints on an entry of loaded procaine, some possible transport paths of which are discussed. |
| |
Keywords: | Procaine Local anesthetic Intracellular pH Intracellular pH regulation pH-selective microelectrode |
本文献已被 SpringerLink 等数据库收录! |
|