两种血小板制品细菌培养结果分析

目的探讨单采血小板与混合血小板在血液采集制备过程中细菌污染的危险程度，为血小板的采集制备过程中改良工艺，提高输血安全性提供可靠数据。方法采用生物梅里埃公司生产的BaeT／Alert3D全自动细菌培养仪及应用需氧瓶（BPA Culture Bottle）、厌氧瓶（BPN Culture Bottle）对5210例单采血小板，1653例混合血小板进行了细菌培养，细菌培养阳性者转种，转种阳性者进行细菌鉴定。结果在5210例单采血小板细菌培养经确定阳性6例，阳性率为1．15‰；1653例混合血小板中细菌培养经确定阳性10例，阳性率6．05‰。结论混合血小板细菌培养阳性率（6．05‰）高于单采血小板（1．15‰），两者比较差异有统计学意义（P〈0．01）；血小板细菌污染情况仍然严峻，应引起注意。

Analysis of bacterial culture results of two types of platelet products

Objective To investigate the criticality of bacterial pollution in the blood collection and preparation process of single donor platelets and mixed platelets in order to provide reliable data for improving the technology in the collection and preparation.process of platelets and improving the blood transfusion safety. Methods The BaeT/ Alert 3D Full-Automatic Bacterial Culture System produced by BioMe rieux and the BPA Culture Bottle and BPN Culture Bottle were used for the bacterial cuhure of 5210 cases of single donor platelets and 1653 cases of mixed platelets.The samples showing g positive bacterial culture results received subcuhivation and the subcuhivated sample showing positive results received bacterial identification. Results Of the 5210 cases of single donor platelets,bacterial culture confirmed 6 positive cases,with the positive rate of 1.15 ‰ .Of the 1653 cases of mixed platelets,bacterial culture confirmed 10 positive cases,with the positive rate of 6.05‰. Conclusion The positive bacterial culture rate of mixed platelets （6.05‰）is higher than that of the single donor platelets （1.15‰）, with statistically significant difference （P 〈 0.01）.The bacterial pollution situation of platelets is still severe and needs attention.