浙江省人禽流感病毒Zhejiang/16/2006株的基因特性分析

目的 分析浙江省首例报道的人禽流感H5N1病毒株A/Zhejiang/16/2006的基因特性与系统进化关系.方法 对A/Zhejiang/16/2006病毒株的全序列8个基因片段作序列测定,并与国内外相关病毒株进行同源性与系统进化比较.结果 A/zhejiang/16/2006的HA序列,与周边国家和地区的H5N1病毒株之间存在11个氨基酸的差异,但这些差异并未影响到相关糖基化位点的稳定;在NA区,1997年以后的毒株均缺少第49～68位的20个氨基酸.总体上中国大陆近年H5N1毒株的8个基因片段与周边国家和地区毒株相比形成另一个分支,并与1996-1997年香港及广东的H5N1毒株之间存在较大差异,但有个别毒株的基因片段在系统进化树上远离当前的毒株而更接近1997年的病毒株.结论 A/zhejiang/16/2006与中国大陆近年的病毒株自成一个体系,与周边国家和地区的H5N1病毒株之间存在一定差异.

Study on the genetic characteristics of the avian influenza virus strain A/Zhejiang/16/2006

OBJECTIVE: To study the genetic characteristics of avian influenza virus strain A/Zhejiang/ 16/2006 which was isolated from the case first reported in Zhejiang province. METHODS: Complete genome of A/Zhejiang/16/2006 including eight segments were sequenced and compared on the genetic homogeneity with sequences of the similar strains provided through domestic and overseas sources. RESULTS: There were 11 amino acids showing differences on HA between A/Zhejiang/16/2006 and the H5N1 isolates of neighboring countries, but these differences had not affected the stability of glycosylation sites. In the NA region, 20 amino acids located in the 49th to 68th position were found absent in the isolates obtained after 1997. Eight segments of H5N1 isolates, circulating in the mainland of China in the recent years, formed a separate branch compared to the strains in neighboring countries and there was also obviously different from the strains isolated in Hong Kong and Guangzhou in 1996 and 1997. However, several Chinese strains were close to the Hong Kong strains isolated in 1997 but diffferent from the current strains in the phylogenetic tree. CONCLUSION: The influenza virus strain A/Zhejiang/16/2006 formed a separate branch with the strains isolated in the mainland of China in the past years but it presented an obvious difference with the isolates from the neighboring countries.