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Temporal relationships and IL-2 dependency of prolactin-induced lymphocyte proliferation |
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| Authors: | Ghennady V Gushchin Carolyn Cheney Ronald Glaser and William B Malarkey |
| Institution: | a Department of Internal Medicine, The Ohio State University Medical Center, N-1106 Doan Hall, 410 West Tenth Avenue, Columbus, OH 43210, USA b Medical Microbiology and Immunology, The Ohio State University Medical Center, 410 West Tenth Avenue, Columbus, OH 43210, USA c Comprehensive Cancer Center, The Ohio State University Medical Center, 410 West Tenth Avenue, Columbus, OH 43210, USA d Behavioral Medicine Research Program, The Ohio State University Medical Center, 410 West Tenth Avenue, Columbus, OH 43210, USA |
| Abstract: | Recombinant preparations of human prolactin (hPRL) and interleukin 2 (hIL-2) as well as monoclonal antibodies to these growth factors were used to study the synergistic interaction of PRL and IL-2 in Nb2 rat lymphoma lactogen-dependent cells. It was shown that IL-2 stimulated Nb2 cell proliferation in lactogen-free culture medium. Experiments with short-term exposure to growth factor demonstrated that PRL was required only during the initial 12 h of incubation while IL-2 was mitogenic regardless of the time it was added. Antibody to IL-2 partially inhibited both PRL- and IL-2-induced proliferation whereas antibody to PRL significantly decreased PRL but not IL-2-induced proliferation. These findings suggest that the complete mitogenic effect of PRL on Nb2 cells requires stimulation of IL-2 production. |
| Keywords: | Prolactin Interleukin 2 Anti-prolactin antibody Anti-interleukin 2 antibody Nb2 lymphoma cells |
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