二十二碳六烯酸对视网膜光感受器细胞超微结构影响的实验研究

目的观察二十二碳六烯酸（DHA）对大鼠视网膜光感受器细胞超微结构的影响。方法 14只雌性SD大鼠随机分成正常组、造模组、DHA组,灌胃15d后造模,分别在造模后12h、24h和48h处死动物,取眼球行电镜观察。结果腹腔内注射一定量的MNU可以明显损伤视网膜光感受器细胞的超微结构。而DHA可抑制MNU对视网膜光感受器细胞超微结构的损伤。结论 DHA对视网膜光感受器细胞有较好的保护作用,有望成为一种延缓视网膜色素变性病程进展的有效药物。

Experimental Study on the Effects of DHA on the Ultrastructure of Photoreceptor Cells

Objective To investigate the effects of DHA on the ultrastructure of photoreceptor cells.Methods Fourteen female SD rats were divided at random into three groups：the normal group,the model group and the DHA group.The model group and the DHA group were administered intragastrically with saline or DHA respectively.After 15 days,retinitis pigmentosa model was made with N-Nitroso-N-methylurea（MNU） and the rats were sacrificed at 12h,24h and 48h after making model,respectively.Eyes were studied with electron microscopy.Results The ultrastructure of photoreceptor cells were obviously injured by intraperitoneal injection of a certain amount of MNU.However,DHA could inhibit the MNU-induced injury of photoreceptor cells.Conclusion DHA has protective effect on photoreceptor cells and may be expected to be an effective drug for preventing the course of retinitis pigmentosa.