HPLC法测定全血中吲达帕胺的浓度

目的建立吲达帕胺全血浓度测定的HPLC法,并用上述方法进行吲达帕胺人全血浓度的测定及药代动力学研究。方法采用Eurospher-100C 150mm×4.6mm I.D.,5μm C18色谱柱;磷酸二氢钠（0.05mol/L,pH3.0）∶甲醇∶异丙醇（54∶40∶6）作流动相,流速：1ml/min;检测波长：241nm;柱温：20℃;以外标法定量。全血样品碱化后加乙酸乙酯萃取,进行HPLC分析。结果本研究建立了吲达帕胺全血浓度测定的HPLC方法,该法在5.0～160.0 ng/ml浓度范围内线性关系良好,r=0.9984,最低定量浓度5.0 ng/ml;精密度试验测得其日内、日间相对标准差（RSD%）均小于6%;低、中、高三个浓度的准确度为100.8%～106.3%;三个浓度的提取回收率为72.6%～80.2%。结论本文建立的测定全血中吲达帕胺浓度的HPLC方法简便易行、方便快捷。

Determination of Indapamide in Human Whole Blood by HPLC

Objective To establish a HPLC method for determination of indapamide in human whole blood.Methods Chromatographic separation was achieved on a Eurospher-100C C18 analytical column.The column temperature was set at 20℃,the mobile phase was composed of phosphate buffer（pH 3.0）∶ methanol∶ isopropyl alcohol（54∶ 40∶ 6）at a flow rate of 1ml/min,and the UV detection wavelength was set at 241nm.The external standard method was used for the analysis.Results The linearity of the method was good within the range of 5.0~160.0 ng/ml,r=0.9984.The lowest detection limit was 5.0 ng/ml,within-day and between-day RSD were less than 6%,the average recovery was over 70%,and the accuracy was within 100.8%~106.3%.Conclusion The established HPLC method is simple rapid and can be used for the study of bioavailability and bioequivalence of indapamide.