| 高效液相色谱法同时测定大豆异黄酮多种组分 |
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| 引用本文： | 贾芸,孙艳芳,张剑峰,王殿文.高效液相色谱法同时测定大豆异黄酮多种组分[J].中国卫生工程学,2009(2):107-108. |
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| 作者姓名： | 贾芸 孙艳芳 张剑峰 王殿文 |
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| 作者单位： | [1]黑龙江省农垦总局红兴隆分局疾病预防控制中心,黑龙江双鸭山155811 [2]黑龙江省疾病预防控制中心,黑龙江双鸭山155811 |
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| 摘 要： | 目的建立同时测定保健食品中大豆异黄酮多种组分的高效液相色谱法。方法样品中的大豆异黄酮采用甲醇＋水提取，经C18柱分离，在紫外检测器254nm波长处检测。结果此方法的回收率为91．9％～102．0％，RSD为2．5％。结论此方法操作简单，准确可靠，为保健食品中大豆异黄酮含量的检测提供参考。
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| 关 键 词： | 大豆异黄酮 液相色谱法 保健食品 |
Simultaneous Detection of Multi - Ingredients of Soybean Isoflavone in Health Food by High Performance Liquid Chromatography |
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| Institution: | JIA Yun, SUN Yan-fang, ZHANG Jian-feng, et al. (Center for Disease Control and Prevention ,Hongxinglong Branch of Land Reclamation Bureau of Heilongfiang Province, Shuangyashan Heilongjiang 155811 , China ) |
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| Abstract: | Objective To establish the method to detect the contents of soybean isoflavone in health food with high performance liquid chromatography （HPLC）. Methods Soybean isoflavone was extracted with methyl alcohol and water. Sample was isolated by C18 pillar, and was detected with 254 nm wavelength of ultraviolet detector. Results The recovery rate of the method was 91.9% - 102. 0% ,and RSD was 2. 5%. Conclusion The method is simple to operate and the result is exact and reliable. The method will provide the reference for content detection of soybean isoflavones in health food. |
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| Keywords: | Soybean isoflavone High performance liquid chromatography Health food |
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