| 光动力学治疗对瘢痕成纤维细胞增殖及其细胞周期的影响 |
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| 引用本文： | 蔡宏,顾瑛,孙强,曾晶,李世龙. 光动力学治疗对瘢痕成纤维细胞增殖及其细胞周期的影响[J]. 中华整形外科杂志, 2010, 26(3). DOI: 10.3760/cma.j.issn.1009-4598.2010.03.014 |
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| 作者姓名： | 蔡宏 顾瑛 孙强 曾晶 李世龙 |
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| 作者单位： | 1. 解放军316医院理疗科,100093
2. 解放军总医院激光医学科 |
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| 摘 要： | 目的 探讨血卟啉单甲醚-光动力学疗法对体外培养瘢痕成纤维细胞的作用.方法 原代培养瘢痕组织来源的成纤维细胞后,建立细胞系,实验采用第4～6代细胞.银染细胞后镜下观察,并计算核仁组成区的相对面积(I.S%);采用流式细胞技术,分析光动力学治疗后各细胞周期中细胞分布比例,并计算增殖指数.结果 ①光动力学治疗后瘢痕成纤维细胞内I.S%显著降低;②流式细胞仪检测结果表明光动力学治疗组S期峰低于对照组,S期细胞比例显著降低,为(11.2±2.3)%;③计算对照组和光动力学治疗组的增殖指数,分别为(35.0±3.4)%和(27.2±3.1)%,两者比较差异有统计学意义(P<0.05).结论 血卟啉单甲醚-光动力学疗法能够抑制瘢痕成纤维细胞的过度增殖,改变其细胞周期分布.
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| 关 键 词： | 光动力学治疗 成纤维细胞 增殖指数 嗜银蛋白 细胞周期 |
Effect of photodynamic therapy on the proliferation and cell distribution of hypertrophic scar fibroblast |
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| CAI Hong,GU Ying,SUN Qiang,ZENG Jing,LI Shi-long. Effect of photodynamic therapy on the proliferation and cell distribution of hypertrophic scar fibroblast[J]. Chinese journal of plastic surgery, 2010, 26(3). DOI: 10.3760/cma.j.issn.1009-4598.2010.03.014 |
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| Authors: | CAI Hong GU Ying SUN Qiang ZENG Jing LI Shi-long |
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| Abstract: | Objective To investigate the effect of HMME-PDT on the proliferation and cell distribution of hypertrophic scar fibroblast (HSF). Methods HSF were cultured and only 4-6th passages were used in this study. Argyrophilic protein in nucleolar organizer regions( AgNORs) were caculated by I. S% after argyrophilic staining. Flow cytometry was applied to analyze the cell cycle and proliferation index (PI). Results ① I. S% of HSF after HMME-PDT was reduced markedly. ② HMME-PDT inhibited HSF entering S stage from G, stage, cell percentage in S stage was decreased to(11. 2 ± 2. 3 ) % . ③ PI in HMME-PDT group was less than that in control group [( 35.0 ± 3.4) % vs (27. 2 ±3. 1)% , P <0.05]. Conclusions HMME-PDT can inhibite proliferation of HSF, and chang cell distribution. |
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| Keywords: | Photodynamic therapy Fibroblasts Proliferation index Argyrophilic protein in nucleolar organizer regions Cell cycle |
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