Genotyping analysis of the MNS blood group system of thalassemia patients with alloantibodies in Iran |
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| Authors: | Atefe Gholamrezazade Naser Amirizadeh Arezoo Oodi |
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| Affiliation: | 1. Unidade de Hemoterapia e Hematologia Samaritano, São Paulo, Brazil;2. Instituto de Tratamento do Câncer Infantil, University of São Paulo School of Medicine, São Paulo, Brazil;3. Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil;1. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia;2. Morozovskaya Children''s Clinical Hospital, Moscow, Russia;1. Blood Bank, Department of Clinical Pathology, University Hospital “Dr. José E. González”, Universidad Autónoma de Nuevo León;2. Liver Unit, Department of Internal Medicine, University Hospital “Dr. José E. González”, Universidad Autónoma de Nuevo León;1. Department of Transfusion Medicine, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi, Mumbai 410210, India;2. Composite Laboratory, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi, Mumbai, 410210, India;1. Department of Hematology and Bone Marrow Transplantation Center, Ankara Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, University of Health Sciences, Ankara, Turkey;2. Department of Clinical Biochemistry, Ankara Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, University of Health Sciences, Ankara, Turkey |
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| Abstract: | BackgroundSerological methods are unreliable for accurate determination of blood group antigens in multi-transfused thalassemia patients. The MNS blood group system has five high-frequency antigens. Many studies demonstrated that some antibodies including anti-S, anti-s, and anti-U may cause acute and delayed transfusion reactions and hemolytic disease of the fetus and newborn. This study aimed to determine the genotype of the MNS blood group in thalassemia patients with alloantibodies by molecular methods.Material and methodsIn this study, 104 blood samples from thalassemia patients were collected. The blood group phenotype for M, N, S and s antigens was determined by the tube hemagglutination method. MNS blood group genotyping was performed using PCR-SSP and DNA Sequencing methods.ResultsAll patients were genotyped with a total of 6 pairs of primers. Discrepancies between genotype and phenotype were observed in 22 patients with S/s alleles and 2 patients with M/N alleles, however, there was full accordance between the results of SSP-PCR and DNA sequencing. The frequency of MNS blood group alleles was determined as follows: 25 % MNSs, 23 % MNss, 21 % MMSs, 9% MMSS, 9% MMss, 8% NNss, 2%MNSS, and NNSS, NNSs, MM genotypes at 1% each.ConclusionIn conclusion, molecular genotyping is more reliable than serological methods in multiple transfusion patients and can lead to a more compatible blood unit for transfusion in these patients. |
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| Keywords: | Genotype Alloimmunization MNS blood group Thalassemia |
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