磷酸化ERK,p38MAPK在婴幼儿血管瘤组织中的表达及意义

目的探讨磷酸化ERK和p38MAPK信号通路在小儿血管瘤增生、消退中的作用。方法应用免疫组化SP法检测47例血管瘤(增殖期26例、消退期21例)组织中磷酸化ERK和p38MAPK的表达情况。结果磷酸化ERK阳性定位于细胞核,其在增殖期和消退期的阳性细胞指数分别为(67.71±12.68)%和(21.54±6.85)%;磷酸化p38MAPK阳性定位于细胞核,其在增殖期和消退期的阳性细胞指数分别为(83.78±5.25)%和(57.79±7.63)%;增生期磷酸化ERK与p38MAPK阳性表达呈正相关,而消退期呈负相关。结论磷酸化ERK和p38MAPK参与血管瘤的增生、消退过程;ERK通路可介导内皮细胞的增生和存活,而p38MAPK可能主要在消退期传递内、外源性凋亡信号,诱导内皮细胞凋亡。

Expression and Biological Significance of Phospho-ERK and p38MAPK in Infantile Hemangioma

Objective To discuss the expression of phospho-ERK,p38MAPK in proliferative and involuting hemangioma of infant.Methods The expression of phospho-ERK,p38MAPK in 47 cases of infantile hemangioma were detected by using immumohistochemistry assay.Results Expression of phospho-ERK protein was located in the nuclei of endotheliocyte.The positive cell index were (67.71±12.68)% and (21.54±6.85)% in proliferativ and involuting hemangioma respectively.Expression of phospho-p38MAPK protein was located in the nuclei of endotheliocyte too.The positive cell index were (83.78±5.25)% and (57.79±7.63)% in proliferative and involuting hemangioma respectively.The expression of phospho-ERK and p38MAPK protein showed positive correlation in proliferative stage of hemangioma,while negative correlation in involuting stage.Conclusion Phospho-ERK,p38MAPK play important role in proliferative and involutional stages of hemangioma.ERK signaling pathway improves survival and proliferation of endotheliocyte,while p38MAPK probably mediates endotheliocyte to apoptosis in endogenous and exogenous stimuli in involutional stage.