| 解脲脲原体和微小脲原体生长特性及分离 |
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| 引用本文： | 程琼,叶元康,陈德利,潘展砚. 解脲脲原体和微小脲原体生长特性及分离[J]. 中国皮肤性病学杂志, 2011, 25(8) |
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| 作者姓名： | 程琼 叶元康 陈德利 潘展砚 |
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| 作者单位： | 1. 同济大学附属东方医院皮肤科,上海,200120
2. 同济大学附属同济医院皮肤性病科,上海,200065 |
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| 摘 要： | 目的探讨解脲脲原体(ureaplasma urealyticum,UU)和微小脲原体(ureaplasma parvum,UP)的生长特性及其分离。方法从妇科门诊随机取266例阴道分泌物标本,通过固、液体培养后应用PCR,基因测序鉴别UU和UP,并筛选出与标准株(ATCC700970)序列相同的UP。取UU标准株T960(CX8=ATCC27618),分别进行培养,绘出生长曲线。结果标本中感染脲原体的有118例,其中UP阳性92例,UU阳性16例,混合阳性10例。固体培养可见大、中、小型的棕色菌落。UU生长周期为22h,UP为30h。液体培养基pH值达到7.5,UU需要13h,UP需要20h。结论临床分离发现UP阳性数高于UU,液体培养中UP的生长周期长于UU,pH值变化速度UP慢于UU,进一步证实了UP和UU是两个不同的种。
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| 关 键 词： | 解脲脲原体 微小脲原体 生长曲线 菌落形态 |
Growth Characteristic and Clinical Isolation Medthod of Ureaplasma Urealyticum and Ureaplasma Parvum |
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| CHENG Qiong,YE Yuan-kang,CHEN De-li,PAN Zhan-yan. Growth Characteristic and Clinical Isolation Medthod of Ureaplasma Urealyticum and Ureaplasma Parvum[J]. The Chinese Journal of Dermatovenereology, 2011, 25(8) |
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| Authors: | CHENG Qiong YE Yuan-kang CHEN De-li PAN Zhan-yan |
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| Affiliation: | CHENG Qiong1,YE Yuan-kang2,CHEN De-li2,PAN Zhan-yan2 (1.Department of Dermatology,East Hospital of Tongji University,Shanghai 200120,China,2.Department of Dermatology,Tongji Hospital of Tongji University,Shanghai 200065,China) |
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| Abstract: | Objective To discuss the growth characteristic and the clinical isolation medthod of Ureaplasma Urealyticum(UU) and Ureaplasma Parvum(UP).Methods Two hundred and sixty-six samples of vaginal secretions were collected from the outpatient department of gynecology randomly.They were cultured by liquid and solid medium.Then PCR technic and sequence analysis were used to distinguish UU from UP.The UP with the same gene sequence as the standard strain(ATCC700970) was selected out.At the same time the standard str... |
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| Keywords: | Ureaplasma Urealyticum Ureaplasma Parvum Growth curve Colony morphous |
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