生物法测定重组葡激酶在股动脉血栓形成模型兔中的药动学参数

目的:研究重组葡激酶(r-SAK)在股动脉球囊损伤后血栓形成模型兔中的药动学。方法:采用自制大块玻璃平板和琼脂井式扩散生物法测定分别静脉不同方式给予r-SAK不同剂量后的兔的血药浓度。结果:r-SAK的药动学特征符合二室模型,血浆检测浓度在20~2500IU/ml范围内与溶圈直径线性关系良好(r=0·9960),平均回收率为(96·05±9·20)%。低、中、高3个剂量静脉滴注组峰浓度分别为(2·28±1·06)、(3·54±0·32)、(6·12±1·61)μg/ml,单次静脉推注组峰浓度为(5·16±1·02)μg/ml。结论:生物法检测r-SAK血药浓度简便、可靠。

Determination of the Pharmacokinetics Parameters of Recombinant Staphylokinase in Thrombosis Model of the Femoral Artery in Rabbits by Biological Assay

OBJECTIVE:To study the pharmacokinetics of recombinant staphylokinase(r-SAK)in a thrombosis model of femoral artery in rabbits.METHODS:Large glass plates were used for modified agar-well diffusion assay to measure r-SAK concentration in plasma of rabbits which had been administered different doses drug by different means respectiveˉly.RERULTS:The pharmacokinetics of r-SAK infusion in thrombosis model of the femoral artery in rabbits fits two-comˉpartment model,the plasma levels and the diameter of lytic circles showed a good linear correlation under the scope of20～2500IU/ml(r=0.9960),and the averaged recovery rate was(96.05±9.20)%.The peak concentrations of low,medial and high dose drop group and single iv group are(2.28±1.06),(3.54±0.32),(6.12±1.61)and(5.16±1.02)μg/ml.CONCLUSION:The biological assays is a simple,reliable method to determine the plasma level.