非小细胞肺癌淋巴转移与nm23—H1基因突变及mRNA表达异…

目的 观察非小细胞肺癌（ＮＳＣＬＣ）组织中ｎｍ２３－Ｈ１基因的存在状况及其转录表达水平，分析肺癌恶性转移与该基因异常的关系。方法 采用ＰＣＲ－ＳＳＣＰ和半定量ＲＴ－ＰＣＲ方法，以正常癌旁组织及正常肺组织为对照，观察了３１例原发性ＮＳＣＬＣ中ｎｍ２３－Ｈ１基因的突变和ｍＲＮＡ表达情况。结果 ３１例肺癌中未发现１例存在ｎｍ２３－Ｈ１基因突变。在２０例具淋巴结转移的ＮＳＣＬＣ中ｎｍ２３－Ｈ１基因ｍＲＮＡ

The correlation between lung cancer lymph node metastasis and nm23-H1 gene mutation, mRNA expression]

OBJECTIVE: To examine the genomic status and the mRNA expression of the nm23-H1 gene, and to analyse the relationship between metastasis of non-small cell lung carcinomas and the gene abnormalities. METHOD: By using PCR-SSCP and semiquantitative RT-PCR, the nm23-H1 gene mutation and mRNA expression were studied in 31 cases of non-small cell lung cancer. The normal tissue adjacent to carcinoma and normal lung tissue were used as control. RESULT: None of the nm23-H1 gene mutation was found in all the lung cancer samples. Decreased expressions of this gene were found in 14 of 20 lung cancer cases with lymph node metastasis, the rate (14/20) of this was significantly higher than that (3/11) of the non-metastatic cancers (P < 0.05). CONCLUSION: These data indicate that nm23-H1 may be a putative metastasis suppressor gene which had shown reverse regulating activation in the metastatic progression of lung cancers, and the level of nm23-H1 mRNA may be considered as one of the pathological indicators in predicting metastatic potential of lung cancers.