An androgen induced hyperinsulinemic and hyperandrogenic anovulatory rat model

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An androgen induced hyperinsulinemic and hyperandrogenic anovulatory rat model

Androgen-induced sterile rats (ASR) were produced by injecting testosterone propionateinto 9 day old SD female rats,Ovarian morphology,hormone levels in the blood and tissues of the pitu-itary,ovary and adrenal,and serum insulin levels were determined.Oral glucose tolerance tests wereperformed.In vitro,testosterone values of thecal-interstitial cells after stimulation by insulin,hCG orinsulin hCG were measured.Results showed that:(1)ovarian size in the ASR were half as big asthat in control rats;(2) pituitary LH concentrations in the ASR were significantly lower than that inthe control group (P<0.01).In the ASR T values in the ovary and the adrenals,and E2 values in theovary were significantly higher than those in the control group(P<0.05 ,P<0.013:(3) in the ASR,glucose tolerance was low and insulin levels high (P<0.01,P<0. 05);(4) T levels were higher in thethecal-interstitial culture medium in the ASR (P<0.001).T levels increased significantly only understimulation by insulin hCG (P<0.001,P<0.01),but not by hCG, nor insulin alone, both in theASR or control rats It suggested that the phenomenon of low LH in the pituitary tissue and high serumT values in ASR might be induced by high serum insulin level in case of insulin resistance. As interac-tion exists between insulin and androgen, ASR may be recognized as a model for hyperinsulinemic andhyperandrogenic anovulation.