重组链激酶腹腔灌注预防大鼠腹腔粘连的实验研究

目的：探讨基因重组链激酶（r-SK）对腹腔粘连的预防价值。方法：30只SD大鼠手术制作腹腔粘连模型后随机分3组，SK组给予r-SK、NS组给予生理盐水腹腔灌注，另一组空白对照（N组），术后1周，2周分别处死动物，观察腹腔粘连程度，并取腹膜和网膜组织进行HE、嗜银染色和纤维蛋白、CD34免疫组化染色，镜下观察组织学改变情况。结果：SK组中，无粘连33.3%，I度粘连61.1%，Ⅱ度粘连5.6%；NS组和N组中均有腹腔粘连，其中2/3表现为Ⅱ～Ⅳ度粘连，镜下表现，腹膜和网膜组织HE，嗜银染色和纤维蛋白，CD34免疫组化染色，显示KS组炎症反应较轻，NS组和N组均呈明显急性炎症反应。结论：r-SK腹腔灌注有减轻大量术后腹腔粘连的作用，有一定的量-效关系。

Experimental study on prevention of the peritoneal adhesion by intracavity perfusion of recombined-streptokinase in rats

Objective:To evaluate the value of recombined-streptokinase(r-SK)in the prevention of peritoneal adhesion.Methods:Peritoneal adhesion model was reconstructed in thirty SD rats.All the rats were divided into SK group,NS group,and N group,and they received intracavity perfusion of r-SK and NS consequently,and none in the N group.One and two weeks after the operation,the animals were sacrificed,and the severity of the peritoneal adhesion was detected.Then the peritoneum in the area of adhesion and the omentum was collected for HE,James's dyeing,and fibrin and CD34 immunohistochemical dyeing.The histological changes were observed under microscope.Results:In SK group,33.3% of the species were normal,61.1% slight adhesion,and 5.6% severe adhesion.In NS group and N group,adhesion was observed in all of the species,and 2/3 was severe.In the SK group,mild inflammatory reaction was revealed,while the abundance of inflammatory exudation and acute inflammatory reaction was observed in the groups of NS and N under the microscope.Conclusion:The peritoneal adhesion in the groups of NS and N were more severe than that of the SK group.r-SK can decrease the peritoneal adhesion significantly,but the efficacy is related to the dosage.