血管内皮生长因子C、D和受体3在人胰腺癌组织中的表达

目的:通过观察不同临床指标的人胰腺癌组织VEGF-C、VEGF-D、VEGFR-3的表达,来探讨VEGF-C和VEGF-D对人胰腺癌转移的影响,为癌组织中淋巴管的生成机制以及癌的淋巴道转移机制提供理论依据。方法:取人胰腺癌标本33例及癌旁正常胰腺组织15例,用免疫组化的方法观察VEGF-C、VEGF-D及VEGFR-3在人胰腺癌和癌旁正常胰腺组织中的表达。结果:VEGF-C、VEGF-D和VEGFR-3在胰腺癌组织中的表达比例较在癌旁正常胰腺组织中的表达比例明显增高,并且VEGFR-3的表达与VEGF-D的表达具有显著相关性(P<0.01),与VEGF-C的表达不具有相关性(P>0.05)。胰腺癌组织中VEGF-C和VEGF-D的表达与患者的年龄、性别、远处转移无关(P>0.05)。结论:VEGF-C、VEGF-D在胰腺癌组织中的表达明显增高,并有可能通过与VEGFR-3的结合促进了癌组织中淋巴管的生成,从而对癌的淋巴道转移起促进作用。

Expression of VEGF-C, VEGF-D and VEGFR-3 in pancreatic carcinoma

Objective:To investigate the role of vascular endothelial growth factor C(VEGF-C), vascular endothelial growth factor D(VEGF-D) and vascular endothelial growth factor receptor 3(VEGFR-3) in pancreatic carcinoma at different conditions, and provide basis for studying the mechanism of tumor lymphagiogenesis and metastasis. Methods: A total of 48 samples were obtained from 33 cases of pancreatic carcinoma and 15 cases of normal pancreatic tissues. The expression of VEGF-C, VEGF-D and VEGFR-3 were analyzed by SP immunohistochemistry. Results: The positive expression of VEGF-C, VEGF-D and VEGFR-3 in pancreatic carcinoma was obviously higher than that in normal pancreatic tissues. The difference existed between the VEGF-D and VEGFR-3 (P<0.01) in pancreatic carcinoma. However, there was no difference between VEGF-C and VEGFR-3, as well between VEGF-C and VEGF-D, despite of age, sex and distant metastases of carcinoma. Conclusions:VEGF-C and VEGF-D express highly in pancreatic carcinoma tissue, which may cause lymphangiogenesis and the metastasis via lymphatics in tumor by combining with its receptor VEGFR-3.