参附注射液对肝脏缺血再灌注大鼠iNOS和NO水平的影响

目的探讨参附注射液(SF)对大鼠肝脏缺血再灌注损伤肝组织中诱导型一氧化氮合酶(iNOS)和血清一氧化氮(NO)的影响。方法32只Wistar大鼠随机分为参附实验组(SF组)和肝缺血再灌注组(IR组)。SF组腹腔注射参附注射液(10ml·kg-1),IR组大鼠给予相同剂量的生理盐水。两组均采用Pringle's法阻断肝门缺血15min再灌注1h、3h,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)以及NO水平,并应用免疫组织化学法测定肝组织中iNOS表达。结果大鼠肝脏缺血15min再灌注1h和3h,SF组血清ALT、AST以及NO水平低于IR组(P<0.05),SF组肝组织iNOS阳性产物平均吸光度值、阳性面积百分率(%)明显低于IR组(P<0.05)。结论参附注射液抑制iNOS的表达,减少过量NO的生成,可能是其对肝脏缺血再灌注损伤的保护作用机制之一。

Influence of ShenFu Injection on the Levels of iNOS and NO in Hepatic Ischemia Reperfusion Rat

Objective To approach the influence of ShenFu(SF)injection on the levels of serum induced nitric oxide synthase(iNOS)and nitric oxide(NO)in hepatic ischemia reperfusion rat.Methods 32 rats were randomly divided into experimental(abdominal injection of SF10ml·kg-1 for 6 days)group and control(NS injection 10ml·kg-1 for 6 days).Pringle's maneuver was used to produce total hepatic ischemia for 15 minutes and reperfuse for 1 hour or 3 hours respectively,contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and NO in serum were measured,the iNOS level in hepatic tissues was determined by immunohistochemistry.Result 1 hour and 3 hours after reperfusion,the levels of ALT,AST and NO in serum in SF experimental group were lower than those in control group(P<0.05),the mean optic density values and positive areas of iNOS were obviously lower in SF experimental group than in control group(P<0.05).Conclusion Inhibition of the expression of iNOS in liver tissues and NO level by SF might be one of mechanisms for SF to protect liver from ischemia reperfusion injury.