| 脑出血大鼠脑组织白介素-10和半胱氨酸蛋白酶-3表达的改变 |
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| 引用本文： | 孙皓,郭富强,王多姿,张红艳.脑出血大鼠脑组织白介素-10和半胱氨酸蛋白酶-3表达的改变[J].临床神经病学杂志,2010,23(1). |
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| 作者姓名： | 孙皓 郭富强 王多姿 张红艳 |
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| 作者单位： | 四川省人民医院神经内科,成都,610072 |
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| 基金项目： | 四川省卫生厅科研基金项目(020019);;四川省中医药管理局科研基金项目(200669) |
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| 摘 要： | 目的 探讨脑出血大鼠脑组织白介素(IL)-10和半胱氨酸蛋白酶(caspase)-3表达的变化.方法 将40只雄性SD大鼠随机分为脑出血1 d组、3 d组、7 d组、14 d组和21 d组,用自体血注入法制作成右侧纹状体脑出血模型.在相应时间点分别给大鼠进行神经功能评分,然后处死大鼠,取右侧脑组织用免疫组化法和原位杂交法检测IL-10和caspase-3蛋白及mRNA阳性细胞数.结果 脑出血1 d组神经功能评分最高,3～21 d组逐渐降低.脑组织IL-10、caspase-3蛋白和mRNA阳性细胞数在脑出血3 d组最多,7～21 d组逐渐减少,21 d组最少;脑出血3 d组与21 d组差异有统计学意义(均P＜0.05);脑出血7 d组IL-10 mRNA和caspase-3蛋白阳性细胞数亦明显多于21 d组,差异有统计学意义(均P＜0.05).结论 脑出血后早期IL-10和caspase-3表达显著增高,随着病程进展逐渐降低,两者的表达可能与预后有关.
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| 关 键 词： | 脑出血 白介素-10 半胱氨酸蛋白酶-3 |
Changes of expression of interleukin-10 and caspase-3 in brain tissue of intracerebral hemorrhage rats |
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| SUN Hao,GUO Fu-qiang,WANG Duo-zi,et al.Changes of expression of interleukin-10 and caspase-3 in brain tissue of intracerebral hemorrhage rats[J].Journal of Clinical Neurology,2010,23(1). |
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| Authors: | SUN Hao GUO Fu-qiang WANG Duo-zi |
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| Institution: | SUN Hao,GUO Fu-qiang,WANG Duo-zi,et al.Department of Neurology,Sichuan Provincial People's Hospital,Chengdu 610072,China |
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| Abstract: | Objective To explore the changes on expression of interleukin(IL)-10 and caspase-3 in brain tissue of intracerebral hemorrhage(ICH)rats.Methods 40 male SD rats were randomly divided into groups of ICH models 1 d,3 d,7 d,14 d and 21 d,and ICH models were made in right striatum by method of injection of self blood.The rats were evaluated by neurological function at the relevant time,and then be killed.The positive cells of protein and mRNA of IL-10 and caspase-3 were detected in the right brain by immunohisto... |
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| Keywords: | intracerebral hemorrhage interleukin-10 caspase-3 |
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