| CO_2对卵巢癌细胞株SKOV3抗凋亡因子XIAP和PED/PEA-15表达的影响 |
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| 引用本文： | 张晓慧,张爱荣,张萍,王亚平,许馥郁. CO_2对卵巢癌细胞株SKOV3抗凋亡因子XIAP和PED/PEA-15表达的影响[J]. 现代妇产科进展, 2007, 16(1): 25-28,I0003 |
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| 作者姓名： | 张晓慧 张爱荣 张萍 王亚平 许馥郁 |
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| 作者单位： | 1. 山东大学第二医院妇产科,济南,250012
2. 山东大学第二医院病理科,济南,250012 |
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| 摘 要： | 目的:探讨CO_2气腹对卵巢癌细胞株SKOV3抗凋亡因子XIAP和PED/ PEA-15表达的影响。方法:建立体外模拟CO_2气腹环境,将人卵巢癌细胞株SKOV3分别暴露于不同压力(8～12mmHg)的CO_2环境中,持续1、2、3h后,脱离CO_2环境,放入常规CO~2培养箱中培养,分别于培养24、48、72h后用实时荧光定量PCR法及免疫细胞化学法检测各组SKOV3细胞XIAP、PED/PEA-15 mRNA和蛋白的表达。结果:SKOV3细胞置于不同的CO_2气腹环境后,与对照组比较,SKOV3细胞XIAP、PED/PEA-15的表达明显增加(P＜0．05),并且与CO_2压力和作用时间有关。结论:CO_2气腹环境可促进SKOV3细胞中抗凋亡因子XIAP、PED/PEA-15表达增加,从而抑制细胞凋亡。
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| 关 键 词： | 卵巢肿瘤 二氧化碳 细胞凋亡 |
| 文章编号： | 1004-7379（2007）01-0025-04 |
| 收稿时间： | 2006-07-02 |
| 修稿时间： | 2006-07-02 |
Effects of carbon dioxide on the expressions of XIAP and PED/PEA-15 in human ovarian cancer cell line SKOV3 |
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| Zhang Xiaohui ,Zhang Airong ,Zhang Ping ,et al.. Effects of carbon dioxide on the expressions of XIAP and PED/PEA-15 in human ovarian cancer cell line SKOV3[J]. Current Advances In Obstetrics and Gynecology, 2007, 16(1): 25-28,I0003 |
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| Authors: | Zhang Xiaohui Zhang Airong Zhang Ping et al. |
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| Affiliation: | 1. Department of Gynecology and Obstetrics;2. Department of Pathology, the Second Affiliated Hospital of Shandong University , Jinan 250012 |
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| Abstract: | Objective:To explore the effects of carbon dioxide on the expressions of antiapoptosis factors XIAP and PED/PEA-15 in human ovarian cancer cell line SKOV3.Meth- ods:Human ovarian cancer cell line SKOV3 was put in analogous CO_2 pneumoperitoneum envi- ronment.Realtime FQ-PCR and immunocytochemical staining were used to examine the expres- sions of XIAP and PED/PEA-15 of these cells after exposure to different pressures and persist- ence of CO_2.Results:CO_2 could significantly increase the expressions of XIAP and PED/PEA- 15(P＜0.05).The expressions of XIAP and PED PEA-15 were related with the pressure and persistence of CO_2.Conclusion:CO_2 can suppress apoptosis capacity of SKOV3 cell by upregu- lating the expressions of antiapoptosis factors XIAP and PED/PEA-15. |
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| Keywords: | Ovarian neoplasms Carbon dioxide Apoptosis |
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