| 糖尿病大鼠早期皮肤改变与血清炎性介质变化 |
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| 引用本文： | 张晓菲,郝飞,杨景.糖尿病大鼠早期皮肤改变与血清炎性介质变化[J].第三军医大学学报,2010,32(8). |
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| 作者姓名： | 张晓菲 郝飞 杨景 |
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| 作者单位： | 1. 第三军医大学西南医院皮肤科,重庆,400038
2. 第三军医大学西南医院病理学研究所,重庆,400038 |
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| 基金项目： | 国家“十一五”攻关项目(2006BA702B01)~~ |
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| 摘 要： | 目的观察糖尿病(diabetes mellitus,DM)大鼠早期皮肤损害的组织病理特点与血清炎性介质的变化。方法将50只8～10周龄Wistar大鼠分为正常对照组(C组,n=25)、DM组(n=25);25只建立DM大鼠模型,其中3只死亡。两组于第4、8、12周末各处死4只,取背部皮肤标本,以观察DM大鼠皮肤早期改变;其余大鼠在第16周末抽取心脏血测定TNF-α、IL-6、C-RP的水平,并处死取皮肤,进行病理学观察及测量皮肤全层与真皮厚度。结果第16周末DM组的TNF-α(1.45±0.67)ng/ml、IL-6(135.05±43.39)pg/ml、C-RP(3.51±0.62)mg/L较正常对照组分别为(0.86±0.60)ng/ml、(99.92±32.36)pg/ml、(2.54±1.31)mg/L]明显增高(P<0.05)。测微尺测量表明DM组大鼠4、8、12、16周皮肤全层均变薄,与正常对照组比较有明显差异(P<0.05,P<0.01)。DM组各时相点表皮厚度较正常对照组薄,但无统计学差异(P>0.05)。组织学观察:DM组表皮细胞层次欠清晰,真皮层胶原萎缩、肿胀、退化变性,皮下脂肪进行性萎...
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| 关 键 词： | 糖尿病 炎症因子 病理 皮肤厚度 |
Early skin changes and serum inflammatory mediators in diabetic rats |
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| Zhang Xiaofei,Hao Fei,Yang Jing.Early skin changes and serum inflammatory mediators in diabetic rats[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(8). |
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| Authors: | Zhang Xiaofei Hao Fei Yang Jing |
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| Institution: | 1Department of Dermatology;2Institute of Pathology;Southwest Hospital;Third Military Medical University;Chongqing;400038;China |
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| Abstract: | Objective To observe the histopathological features of early skin lesions and serum inflammatory mediators changes in the diabetes mellitus(DM) rats.Methods A total of 50 Wistar rats at 8 to 10 weeks of age were divided into normal control group(C group,n=25) and diabetic group(DC group,n=25).The rats of DC group were given a high-glucose and high-fat diet for 2 weeks to induce insulin resistance and then an intraperitoneal injection of 35 mg/kg streptozotocin.All 25 rats were identified to be diabetic mode... |
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| Keywords: | diabetes mellitus inflammatory factor pathology skin thickness |
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