RNA-Linked DNA Fragments In Vitro |
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| Authors: | Akio Sugino and Reiji Okazaki |
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| Institution: | Institute of Molecular Biology, Faculty of Science, Nagoya University, Nagoya, Japan 464 |
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| Abstract: | RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch to the 5' end of the DNA is proved by transfer of (32)P from alpha-(32)P]dNTP to ribonucleotides upon digestion with alkali or pancreatic RNase, and by a small decrease in the molecular size upon alkaline hydrolysis. The (32)P transfer experiments reveal a unique structure...p(rPy)p(rA)p(rU or rC)p(dC)p... at the RNA-DNA junction. |
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