| Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells |
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| 引用本文： | Hao Cheng,Zhongyao Wu,Jianliang Zheng,Guilan Lu,Jianhua Yan Min Liu,Danping Huang,Jianxian LinZhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China. Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells[J]. 眼科学报, 2003, 19(1): 54-59 |
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| 作者姓名： | Hao Cheng Zhongyao Wu Jianliang Zheng Guilan Lu Jianhua Yan Min Liu Danping Huang Jianxian LinZhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China |
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| 作者单位： | Hao Cheng，Zhongyao Wu，Jianliang Zheng，Guilan Lu，Jianhua Yan Min Liu，Danping Huang，Jianxian LinZhongshan Ophthalmic Center，Sun Yat-sen University，Guangzhou 510060，China |
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| 摘 要： | Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma. Material and Methods: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telormarse was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.Results: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24h and 1μg/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melan
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| 关 键 词： | 端粒酶 细胞毒素 脉络膜黑色素瘤细胞 顺铂 细胞培养 PCR-ELISA |
Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells |
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| HAO Cheng,Zhongyao Wu,Jianliang Zheng,Guilan Lu,Jianhua Yan,Min LIU,Danping Huang,Jianxian Lin. Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells[J]. Eye science, 2003, 19(1): 54-59 |
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| Authors: | HAO Cheng Zhongyao Wu Jianliang Zheng Guilan Lu Jianhua Yan Min LIU Danping Huang Jianxian Lin |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China |
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| Abstract: | PURPOSE: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma. MATERIAL AND METHODS: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telomerase was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis. RESULTS: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24 h and 1 microgram/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase. CONCLUSIONS: CDDP is an effective telomerase inhibitor in cultured choroidal melanoma cells of human eyes, which presents concentration and time dependency and can cause the death of cultured cells. |
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| Keywords: | cisplatin choroidal melanoma telomerase cytotoxic effect |
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