何首乌不同炮制品对大鼠血虚模型的补血作用

目的:考察何首乌不同炮制品对大鼠血虚模型的补血作用并探讨其最佳炮制方法。方法:140只SD大鼠,随机分为空白组、模型组、阳性药物组和11个不同何首乌炮制品组,每组10只,分别灌胃给予蒸馏水、血宝补血冲剂(阳性对照药)和不同何首乌炮制品(剂量均为生药含量2 g·kg-1),每天1次,连续10天,于第10天时取血检查各组动物红细胞、红血球蛋白、红细胞压积、全血和血浆黏度和凝血酶原时间等指标。结果:何首乌常压豆制24 h、常压酒豆制24 h、高压清蒸4 h和8 h、高压豆制6 h和8 h组、高压酒制6 h以及高压酒豆制8 h组能显著增加血虚大鼠模型的红细胞数量和血红蛋白数量,其中高压豆制何首乌8 h组红细胞和血红蛋白数增加效果最佳(P<0.01)。生何首乌、常压豆制24 h、酒制24 h、酒豆制24 h,高压清蒸4 h和8 h、高压豆制6 h和8 h以及高压酒制6 h能够改善血虚大鼠模型的血流变功能紊乱(P<0.05或P<0.01)。结论:何首乌以黑豆汁或酒为辅料,高压蒸制6~8 h,补血效果更好。

Blood-enriching Effects of Different Processed Radix Polygoni Multiflori Slices on Blood Deficiency Rats

This study was aimed to investigate the blood-enriching effect of processed Radix Polygoni Multiflori on blood deficiency rats. A total of 140 SD rats were randomly divided into 14 groups, which were the blank control group, blood deficiency model group, positive control group and 11 test groups. The blank control group and model control group were treated with distilled water and the positive control group was given Xuebao Buxue soluble granules. The test groups were treated with 11 different kinds of processed Radix Polygoni Multiflori samples at a dosage of 2 g·kg-1 respectively. All rats were given drugs once a day. After 10 days, blood samples were collected and the amount of red blood cell (RBC), hemoglobin (Hb), haematocrit (HCT), whole blood viscosity and plasma viscosity, prothrombin time were estimated. The results showed that 9 processed Radix Polygoni Multiflori groups can increase the number of RBC and Hb significantly (P < 0.05 or P < 0.01) in blood deficiency rat models. They were the sample processed with decoction of black soybean (DBS) for 24 h, with wine and DBS combination for 24 h, steamed for 4 h and 8 h under high pressure, with DBS for 6 h and 8 h, with wine for 6 h, with wine and DBS combination under high pressure, respectively. The samples processed with DBS under high pressure for 8 h increased RBC and Hb were more obviously (P < 0.01). The 8 groups can improve the situation of functional disorder of blood rheology significantly (P < 0.05 or P < 0.01). They were the unprocessed samples, processed with DBS and wine for 24 h respectively, with DBS and wine combination for 24 h, steamed for 4 h and 8 h or steamed with DBS for 6 h and 8 h or with wine for 6 h under high pressure respectively. It was concluded that the blood-enriching effect was improved while the Radix Polygoni Multiflori was processed with DBS or wine and under high pressure for 6-8 h.