交泰丸对血管性痴呆模型大鼠学习记忆能力及脑组织胆碱能机制的影响

目的研究交泰丸对血管性痴呆(VD)大鼠学习记忆及脑组织胆碱能机制的影响,探讨其对VD治疗的作用及机制。方法采用线栓法制备VD大鼠模型,MG-2Y迷宫进行行为学测试,羟胺比色法测定脑组织乙酰胆碱(Ach)含量,分光光度法测定脑组织乙酰胆碱酯酶(AchE)活性,HE染色观察海马区病理变化。结果模型对照组大鼠学习记忆能力明显劣于假手术组,Ach含量降低,AchE活性升高,海马区病理改变严重;交泰丸治疗后,尤其是高剂量交泰丸组,学习记忆能力明显提高,Ach含量升高,AchE活性降低,海马区病理变化明显改善。结论交泰丸对VD大鼠学习记忆能力下降有明显的保护作用,其作用机制可能与抑制AchE活性,提高Ach含量,修复及保护缺血再灌注VD大鼠海马区神经元密切相关。

The effects of Jiaotai pill on vascular dementia rat model of learning and memory ability and brain cholinergic mechanisms

Objective Research the impact of Jiaotai pill on the ability of learning,memory and the mechanism of brain cholinergic.Discuss the function and mechanism of Jiaotai pill for VD therapy.Methods Use the intraluminal method of Gong Biao to prepare the model of VD rats.MG-2Y maze did the test of behavior science.Use Hydroxylamine colorimetric method to examine the content of Ach in brain.Use the spectrophotometric method to examine the activity of AchE in brain.After HE staining,observed the changes of Hippocampus pathological.Results Learning and memory ability of model control group rats obviously was inferior to surgery group,Ach proportion reduced,AchE activity rised,and pathological changes in hippocampus were serious.After Jiaotai pill treatment,especially group of the used high Jiaotai pill,the ability of learning and memory obviously increased,Ach proportion increases,AchE activity reduced,Hippocampus pathological changes obviously improved.Conclusion Jiaotai pill can protect the activity of learning and memory for VD rats clearly.The effect mechanism may be closely connect with restraining the activity of AchE,increasing the content of Ach,restoration and protection in neurons in hippocampus of rats with ischemia reperfusion VD.