| 果糖二磷酸钠片含量测定方法研究 |
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| 引用本文： | 金兴成,刘丽萍.果糖二磷酸钠片含量测定方法研究[J].安徽医药,2012,16(1):44-45. |
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| 作者姓名： | 金兴成 刘丽萍 |
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| 作者单位： | 安徽医科大学第二附属医院药剂科,安徽,合肥,230601;安徽医科大学第二附属医院药剂科,安徽,合肥,230601 |
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| 摘 要： | 目的 建立果糖二磷酸钠片含量测定方法.方法 采用酶法测定含量,通过使用还原型烟酰胺腺嘌呤二核苷酸(NADH)、3-磷酸甘油脱氢酶-磷酸丙糖异构酶(GDH-TIM)﹑醛缩酶(ALD)催化果糖二磷酸钠降解,在340 nm波长处测定其吸收度.结果 果糖二磷酸钠在0～400 mg· L-1范围内呈良好的线性关系(r=0.999 5 ),平均回收率为99.04%(RSD=0.57%,n=9).结论 该方法灵敏﹑准确,重复性好,能满足果糖二磷酸钠片含量测定的要求.
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| 关 键 词： | 果糖二磷酸钠片 含量测定 pH |
Determination of fructose diphosphate sodium tablets |
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| JIN Xing-cheng,LIU Li-ping.Determination of fructose diphosphate sodium tablets[J].Anhui Medical and Pharmaceutical Journal,2012,16(1):44-45. |
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| Authors: | JIN Xing-cheng LIU Li-ping |
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| Institution: | (Department of Pharmacy,The Second Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230601,China) |
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| Abstract: | Objective To establish a method for the determination of fructose diphosphate sodium tablets.Methods The content of FDP-Na was determined by enzymatic method.By using reduced niacinamide adenine dinucleotied（NADH）﹑glycerophosphate dehydrogenase-triosephosphosphate（GDH-TIM） and aldolase（ALD） to catalyze the degradation of FDP-Na,measuring the absorbance at wavelength of 340 nm.Results The calibration curves was linear in the range of 0~400 mg·L-1for fructose diphosphate sodium tablets（r=0.999 5）.The average recovery was 99.04%（RSD=0.57%,n=9）.Conclusion The determination method is sensitive,accurate and repetitive.It can satisfy the determination of fructose diphosphate sodium tablets. |
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| Keywords: | fructose diphosphate sodium tablets content determination pH |
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