| HPLC法测定丙酸氟替卡松乳膏中丙酸氟替卡松的含量 |
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| 引用本文： | 赵艳,张虹,张霄翔. HPLC法测定丙酸氟替卡松乳膏中丙酸氟替卡松的含量[J]. 安徽医药, 2012, 16(1): 33-35 |
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| 作者姓名： | 赵艳 张虹 张霄翔 |
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| 作者单位： | 合肥工业大学制药工程系,安徽,合肥,230009;安徽省医药科技实业公司,安徽,合肥,230001 |
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| 摘 要： | 目的 建立丙酸氟替卡松乳膏中丙酸氟替卡松含量测定的方法.方法 采用高效液相色谱法,色谱柱为C18柱(250 mm×4.6 mm,5 μm),流动相为0.02 M磷酸溶液(三乙胺调pH=4.5)-甲醇(24:76),流速1.0 ml·min-1,检测波长为240 nm,柱温35℃.结果 丙酸氟替卡松进样浓度在10～35 mg·L-1范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为98.83%,RSD为0.47%(n=9).结论 测定方法专属性强、精密、准确、灵敏、简便可行,可作为丙酸氟替卡松乳膏的质量控制方法.
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| 关 键 词： | 丙酸氟替卡松乳膏 丙酸氟替卡松 高效液相色谱法 含量测定 |
Determintion of fluticasone propionate in fluticasone propionate cream by HPLC |
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| ZHAO Yan,ZHANG Hong,ZHANG Xiao-xiang. Determintion of fluticasone propionate in fluticasone propionate cream by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2012, 16(1): 33-35 |
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| Authors: | ZHAO Yan ZHANG Hong ZHANG Xiao-xiang |
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| Affiliation: | 1.Department of Pharmaceutical Engineering,Hefei University of Technology,Hefei 230009,China; 2.Anhui Medical Technology Industrial Company,Hefei 230001,China) |
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| Abstract: | Objective To establish a method for the determination of fluticasone propionate in fluticasone propionate cream.Methods The C18 column was used with the mobile phase of 0.02 M phosphoric acid solution（adjusted to pH=4.5 with triethylamine）-methanol（24∶ 76） and with the flow rate of 1.0 ml·min-1 at the detection wavelength of 240nm.The detection temperature was 35℃.Results The calibration curve for fluticasone propionate was linear in the concentration ranges of 10~35 mg·L-1（r=0.999 9）.The average recovery was 98.83%,and RSD was 0.47%（n=9）.Conclusion This assay is specific,precise,accurate,sensitive and could be used as the method of quality control of futicasone propionate cream. |
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| Keywords: | fluticasone propionate cream fluticasone propionate HPLC determination |
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