| 多重耐药鲍曼不动杆菌外排泵机制的初步研究 |
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| 引用本文： | 王友梅,沈继龙,沈继录,徐元宏.多重耐药鲍曼不动杆菌外排泵机制的初步研究[J].安徽医科大学学报,2012,47(1):38-40. |
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| 作者姓名： | 王友梅 沈继龙 沈继录 徐元宏 |
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| 作者单位： | 安徽医科大学第一附属医院检验科,合肥,230022;安徽医科大学安徽省部共建重要遗传病基因资源利用重点实验室,合肥,230032 |
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| 摘 要： | 目的研究外排泵在多重耐药鲍曼不动杆菌中的存在情况,为进一步研究其耐药机制奠定基础。方法采用M-H琼脂稀释法,测定添加和不添加抑制剂苯丙氨酸-精氨酸-β-萘胺(PAβN)前后鲍曼不动杆菌对亚胺培南、美罗培南、庆大霉素、环丙沙星和头孢他啶5种药的最低抑菌浓度(MIC)。结果添加PAβN后,74株多重耐药鲍曼不动杆菌对5种药的MIC值下降4倍以上的菌株数分别为亚胺培南45(60.81%)株、美罗培南70(94.59%)株、庆大霉素0(0%)株、环丙沙星4(5.41%)株、头孢他啶45(60.81%)株。结论外排泵机制可能是引起鲍曼不动杆菌对亚胺培南、美罗培南、头孢他啶耐药的主要机制;外排泵机制对环丙沙星和庆大霉素耐药不起主要作用。
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| 关 键 词： | 鲍曼不动杆菌 多重耐药 外排泵 PAβN |
Primary study on efflux pump mechanism in multidrug-resistant Acinetobacter baumannii |
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| Institution: | Wang Youmei1,Shen Jilong2,Shen Jilu1,et al(1Dept of Medical Laboratory,The First Affiliated Hospital of Anhui Medical University,Hefei 230022; 2The Key Laboratory of Gene Resource Utilization for Severe Diseases,Anhui Medical University,Hefei 230032) |
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| Abstract: | Objective To study the phenotype of the efflux pumps in multidrug-resistant Acinetobacter baumannii providing foundation for further study the mechanism of multidrug-resistant in Acinetobacter baumannii.Methods The minimum inhibitory concentrations(MIC) of five antibiotics,including imipenem,meropenem,ciprofloxacin gentamicin and ceftazidime with or without efflux pump inhibitor PAβN were determined by agar dilution method.Results In addition of PAβN,MIC of 45 strains to imipenem,70 strains to meropenem,45 strains to ceftazidime,only 4 strains to ciprofloxacin decreased more than 4 folds,but no one strain to gentamicin.Conclusion Efflux pump mechanism is one of the causes of imipenem,meropenem and ceftazidime resistant in Acinetobacter baumannii,while which is not account for ciprofloxacin and gentamicin. |
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| Keywords: | Acinetobacter baumannii multidrug resistance efflux pump PAβN |
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