Tyrosine phosphatase SHP-1 is expressed higher in multisystem than in single-system Langerhans cell histiocytosis by immunohistochemistry |
| |
Authors: | Murakami Ichiro Oka Takashi Kuwamoto Satoshi Kato Masako Hayashi Kazuhiko Gogusev Jean Imamura Toshihiko Morimoto Akira Imashuku Shinsaku Yoshino Tadashi |
| |
Institution: | (1) Faculty of Medicine, Division of Molecular Pathology, Tottori University, 86 Nishi-cho Yonago-shi, Tottori 683–0834, Japan;(2) Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;(3) Inserm U90 and U507, H?pital Necker-Enfants-Malades, Paris, France;(4) Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan;(5) Department of Pediatrics, Jichi Medical University School of Medicine, Shimotsuke, Japan;(6) Division of Pediatrics and Hematology, Takasago-seibu Hospital, Takasago, Japan |
| |
Abstract: | Langerhans cell histiocytosis (LCH) is a proliferative disorder of Langerhans cell (LC)-like CD1a-positive cell (LCH cell)
with unknown causes. LCH consists of two subtypes: single-system LCH (LCH-SS) with favorable prognosis and multisystem LCH
(LCH-MS) with poor prognosis. LCH has been indicated as a neoplastic disorder from monoclonal characteristics of LCH cells.
This study aimed to investigate an expression of tyrosine phosphatase SHP-1 in LCH, since its expression levels were variously
reported in many tumors, overexpression in ovarian cancers (a candidate oncoprotein), and downregulation by methylation in
gastric cancers, prostate cancers, malignant lymphomas, and leukemias (a putative tumor suppressor). By immunohistochemistry
(IHC), the SHP-1 expression in LCs and LCH cells was compared in LCH (two subtypes: LCH-SS = 21, LCH-MS = 12), dermatopathic
lymphadenopathy (DLA) (n = 9) and normal epidermal LCs (n = 3) near LCH lesion. IHC results were analyzed semiquantitatively using a Photoshop software. The mean intensity score (IS)
of DLA, LCH-SS, LCH-MS, and LCs were 47, 100, 139, and 167 (in arbitrary unit), respectively. The IS had significant differences
among LCH-SS, LCH-MS, and DLA (p < 0.01). SHP-1 is expressed significantly higher in LCH-MS than in LCH-SS. SHP-1 can be a progression marker of LCH. SHP-1
is also useful for differential diagnosis between LCH in lymph nodes and DLA. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|