正常人外周血单核细胞基因表达谱的建立及临床意义

目的:建立正常人外周血单核细胞的基因表达谱,探讨颅内动脉瘤的差异基因,为研究颅内动脉瘤的发病机制及其可能的基因治疗打下基础。方法:分别抽提60例正常人和颅内动脉瘤患者外周血单核细胞的RNA,基因芯片技术检测其外周血中的基因表达情况。结果:在基因芯片所检测的共14112个基因中,有7749个基因在正常人外周血单核细胞中呈阳性表达,表达数量较多的基因分别是:细胞信号和传递蛋白表达类、代谢相关类、离子通道和运输蛋白、结构运动类基因。表达水平位于前3位的基因分别是核糖体蛋白L41(RPL41)、核糖体蛋白S3A(RPS3A)和核糖体蛋白L23A(RPL23A)基因。与正常人外周血阳性基因比较,颅内动脉瘤患者得到85个差异基因,其中KPNA4基因与信号传导、蛋白质转运过程功能有关,基因表达明显上调;SPG3A基因与核苷酸结合功能相关,基因表达明显下调。结论:建立了正常人外周血的基因表达谱,KPNA4基因和SPG3A基因可能与颅内动脉瘤形成和发展有关。

Establishment and Clinical Significance of the Gene Expression Map of Normal Peripheral Blood Mononuclear Cells

Objective:To establish the gene expression map of normal peripheral blood mononuclear cells and to study the mechanism and the gene therapy of intracranial aneurysm(IA).Methods:The total RNA was collected from 60 normal people and patients with IA.The gene chip technology was used to analyze the genetic expression of intracranial arterial blood.Results:The gene chip was used to detect 14 112 genes.There were 7 749 genes expressed in the normal peripheral blood.The gene expression levels were more in gene/protein expression categories,metabolism category and the structure/ movement genome.Of which,L41(RPL41),S3A(RPS3A) and L23A(RPL23A) were the first third of the gene expression.Com-pared to IA patients,there were 85 differently expressed genes,the KPNA4 gene expression was up-regulated,which was relat-ed to signal conduction and protein transmission.The SPG3A gene expression was down-regulated,which was related to nu-cleotide conjunctin.Conclusion:The gene expression map of the normal peripheral blood was established.KPNA4 and SPG3A may be related to the development of IA.