独活挥发油成分的GC-MS分析

目的:分析我国中药材GAP基地之一所种植独活挥发油的化学成分,为其质量评价提供科学依据。方法:采用水蒸气蒸馏法提取独活挥发油,用GC毛细管柱进行分析,归一化法测定其相对含量,并用GC-MS法鉴定化学成分。结果:检出229个色谱峰,鉴定出88个化合物,占挥发油总量的78.23%。结论:独活挥发油的单萜类化合物及其衍生物中,3-蒈烯(8.89%)、间-聚伞花素(4.99%)、β-水芹烯(8.35%)等是主要成分,占总挥发油量的22.23%;倍半萜类化合物及其衍生物中,桉叶烷-4(14),11-二烯(4.36%)、α-芹子烯(1.36%)、喇叭醇(1.14%)、α-甜没药萜醇(6.03%)和1,8-二甲基-4-异丙基-螺环4.5]+-8-烯-7-酮(4.37%)等是主要成分,占总挥发油量的17.26%。

GC-MS analysis of essential oils from Radix Angelicae Pubescentis

OBJECTIVE: To analyze the constituents of the essential oils from Radix Angelicae Pubescentis planted in a GAP base of the Chinese medica materials, and provide the scientific basis for quality control. METHOD: The essential oils were extracted by water-steam distillation and separated by GC capillary column chromatography. The components were calculated with normalization method, and were identified by MS detector. RESULT: 229 peaks weredetected, among which 8 compounds have been identified, which made up 78.23% of the total essential oil. CONCLUSION: In the total essential oils of Radix Angelicae Pubescentis, 3-carene (8.89%), m-cymene (4.99%) and beta-phellandrene (8.35%) as the monoterpenes and its derivatives, and eudesma-4 (14), 11-diene (4.36%), alpha-selinene (1.36%), ledol (1.14%), alpha-bisabolol (6.03%), and 1, 8-dimethyl-4-(1-methylethyl)-spiro4.5]dec-8-en-7-one (4.37%) as the sesquiterpenes and its derivatives, are main components.