肝细胞癌组织中HBV，HCV，HGV的表达

目的　探讨乙型肝炎病毒 (HBV) ,丙型肝炎病毒 (HCV)及庚型肝炎病毒 (HGV)在肝细胞癌 (HCC)组织中的存在状态。方法　分别采用抗 -HBVS ,抗 -HCVNS5及抗 -HGVNS5三种单克隆抗体 ,以免疫组织化学SPAB法检测 2 6例HCC患者石蜡包埋的肝癌及癌旁肝组织中HBVS抗原 (HBsAg) ,HCVNS5抗原 (HCAg)和HGVNS5抗原(HGAg)。结果　检出HBsAg 17例 ( 65 % ) ,HCAg 13例 ( 5 0 % )及HGAg 9例 ( 3 5 % ) ,其中HBV/HCV/HGV重叠感染 7例 ,HBV/HCV重叠感染 4例 ,HBV单独感染 6例 ,HCV单独感染 2例 ,HGV单独感染 2例 ,另有 5例均不表达上述三种抗原 ;HBsAg与HGAg阳性率之间差异有显著性 (P <0 0 5 ) ,HBsAg与HCAg及HCAg与HGAg阳性率之间差异均无显著性(P >0 0 5 ) ;HBV ,HCV及HGV均表达于肝细胞或肝癌细胞浆内 ,呈细颗粒状分布 ,癌组织表达强度均弱于癌旁组织。结论　HCC患者中有较高的HBV ,HCV及HGV感染率 ,感染率顺序为HBV >HCV >HGV ;推测HBV感染在HCC发生过程中起重要作用 ,HCV和HGV感染可能是HCC发生的相关因素。

Hepatitis viruses in hepatocellular carcinoma

Objective To investigate the pattern of hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis G virus (HGV) in tissues of hepatocellular carcinoma (HCC) and para-HCC. Methods HBV S antigen (HBsAg), HCV NS5 antigen (HCAg) and HGV NS5 antigen (HGAg) were detected respectively in paraffin-embedded HCC tissues from 26 cases of patients with HCC by using immunohistochemical method (SPAB) with three monoclonal antibodies of anti-HBs, anti-HCV NS5 and anti-HGV NS5. Results The positive rate of HBsAg, HCAg and HGAg was 65%(17/26), 50%(13/26) and 35%(9/26), respectively. There were overlapping infection of HBV/HCV/HGV in 7 cases and HBV/HCV in 4 cases. 6 cases of HBV, 2 cases of HCV and 2 cases of HGV were all single infection. 5 cases were negative for three antigens. there were significant difference between HBV and HGV infection and no significant difference between HBV and HCV, HCV and HGV infection. All three antigens expressed in cytoplasma of liver or HCC cells, in thin granular pattern. Expression of the three antigens in HCC cells was weaker than that in para-HCC cells. Conclusion There are high infection rates of HBV, HCV and HGV in patients with HCC, in the order of HBV, HCV and HGV. HBV infection may play an important role, in the development of HCC while HCV and HGV infecction are related factors.