应用荧光原位杂交技术检测肺癌针吸标本中超二倍体肿瘤细胞

目的：探讨肺癌针吸标本中肿瘤细胞染色体数目的异常变化。方法：用７号、１１号、１７号、Ｘ梁色体特异的着丝粒ＤＮＡ探针,对３０例肺癌针吸标本（６例为肺癌原发灶标本,２４例为颈淋巴结标本）进行双色荧光原位杂交。结果：２７例肺癌阳性针吸标本中７号、Ｘ、１７号、１１号染色体超二倍体的病例分别达到８１．５％（２２／２７）、７７．８％（２１／２７）、７０．４％（１９／２７）、６３．０％（１７／２７）。３例肺癌阴性针吸标本中４个染色体均未出现超二倍体改变。结论：⑴用荧光原位杂交技术对针吸标本进行细胞遗传学分析是一种可行、简单的方法。⑵肺癌针吸标本中肿瘤细胞染色体超二倍体改变对诊断良、恶性细胞具有一定的参考价值。

Detection of hyperdiploid in malignant cells in fine-needle aspirates from lung cancer by fluorescence in situ hybridization

OBJECTIVE: To study the numerical abnormality of chromosomes in malignant cells in fine-needle aspirates from lung cancer by fluorescence in situ hybridization (FISH). METHODS: 30 fine-needle aspirates from lung cancer (4 from lung tissues, 26 from lymph nodes) were detected by FISH with the centromere DNA probes for chromosome 7, 11, 17 and X. RESULTS: In 27 positive fine-needle aspirates the rates of hyperdiploid in chromosome 7, X, 17, and 11 were 81.5% (22/27), 77.8% (21/27), 70.4% (19/27) and 63.0% (17/27), respectively. In 3 negative fine-needle aspirates, the number of chromosomes was normal. CONCLUSIONS: (1) Interphase cytogenetic cells analysis of fine-needle aspirates by FISH is feasible and simple; (2) Hyperdiploid in malignant cells in fine-needle aspirates from lung cancer can be used as a biomarker for benign and malignant cells.