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Human papillomavirus DNA in head and neck squamous cell carcinomas in the Free State,South Africa |
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| Authors: | Atang Bulane MSc Dominique Goedhals PhD Riaz Y. Seedat MMed FCORL (SA) Jacqueline Goedhals MMed Felicity Burt PhD |
| Affiliation: | 1. Division of Virology, University of the Free State, Bloemfontein, South Africa;2. Division of Virology, University of the Free State, Bloemfontein, South Africa Division of Virology, National Health Laboratory Service, Bloemfontein, South Africa;3. Department of Otorhinolaryngology, University of the Free State, Bloemfontein, South Africa;4. Department of Anatomical Pathology, University of the Free State, Bloemfontein, South Africa Department of Anatomical Pathology, National Health Laboratory Service, Bloemfontein, South Africa |
| Abstract: | Human papillomaviruses (HPVs) have been associated with a subset of head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to determine the prevalence of HPV DNA in archived formalin-fixed paraffin-embedded tissue from patients with histologically confirmed HNSCCs in a South African cohort. A nested PCR was used for the detection of HPV DNA targeting the L1 gene. Positive samples were confirmed using an in-house hemi-nested PCR targeting the E6 gene and genotyped by sequence determination of amplicons. HPV DNA was detected in 57/780 (7.3%) samples, with the highest prevalence being in the sinonasal tract (16.0%) and oropharynx (10.8%). HPV16 was the most frequently detected type, being found in 26/57 (45.6%) positive samples. The prevalence of HPV DNA in HNSCCs found in this study was lower than that found in developed countries |
| Keywords: | formalin-fixed paraffin-embedded tissues head, and neck squamous cell carcinomas human papillomaviruses hypopharynx carcinoma larynx carcinoma nasopharynx carcinoma oral squamous cell carcinoma oropharynx carcinoma prevalence sinonasal carcinoma |