Radiolabeled hormones in insulin research,a minireview |
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Authors: | Jiří Jiráček Lenka Žáková Aleš Marek |
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Institution: | Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Prague, Czech Republic |
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Abstract: | Preparation of both 125I-labeled insulin and insulin-like growth factor 1 (IGF-1) was critical because it enabled a detailed characterization of binding properties of these important hormones towards their cognate transmembrane receptors. Binding modes of hundreds of hormone derivatives were analyzed using competition radioligand binding assays. This effort has resulted in development of six insulin analogs that are today clinically used for the treatment of diabetes. Here, we will briefly summarize a history of insulin research employing iodinated hormones. |
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Keywords: | assay binding hormone IGF insulin |
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