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Preeclampsia enhances neuroglial marker expression in umbilical cord Wharton's jelly-derived mesenchymal stem cells |
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| Authors: | Marianne Joerger-Messerli Anna Wagner Martin Mueller |
| Institution: | 1. Department of Obstetrics and Gynecology, University Hospital Bern, Bern, Switzerland and;2. Department of Clinical Research, University of Bern, Bern, Switzerland;3. Department of Obstetrics and Gynecology, University Hospital Bern, Bern, Switzerland and |
| Abstract: | Objective: The aim of the study was to compare the neuroglial phenotype of Wharton's jelly-derived mesenchymal stem cells (WJ-MSC) from pregnancies complicated with preeclampsia and gestational age (GA)-matched controls. Methods: WJ-MSC were isolated from umbilical cords from both groups and analyzed for the cell surface expression of MSC markers and the gene and protein expression of neuroglial markers. Results: All WJ cells were highly positive for the MSC markers CD105, CD90 and CD73, but negative for markers specific for hematopoietic (CD34) and immunological cells (CD45, CD14, CD19 and HLA-DR). WJ-MSC from both groups expressed neuroglial markers (MAP-2, GFAP, MBP, Musashi-1 and Nestin) at the mRNA and protein level. The protein expressions of neuronal (MAP-2) and oligodendrocytic (MBP) markers were significantly increased in WJ-MSC from preeclampsia versus GA-matched controls. Conclusions: WJ-MSC from preeclamptic patients are possibly more committed to neuroglial differentiation through the activation of pathways involved both in the pathophysiology of the disease and in neurogenesis. |
| Keywords: | Mesenchymal stem cells neuroglial differentiation perinatal brain injury preeclampsia Wharton's jelly |