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Amniotic fluid markers of oxidative stress in pregnancies complicated by preterm prelabor rupture of membranes |
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| Authors: | Marian Kacerovsky Lubomira Tothova Ramkumar Menon Barbora Vlkova Ivana Musilova Helena Hornychova |
| Affiliation: | 1. Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic,;2. Department of Obstetrics and Gynecology, Faculty of Medicine Hradec Kralove, Charles University in Prague, Prague, Czech Republic,;3. Institute of Molecular Biomedicine, Comenius University in Bratislava, Bratislava, Slovak Republic,;4. Center for Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovakia,;5. Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, USA,;6. Department of Obstetrics and Gynecology, Faculty of Medicine Hradec Kralove, Charles University in Prague, Prague, Czech Republic,;7. Fingerland’s Department of Pathology, Faculty of Medicine Hradec Kralove, Charles University in Prague, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic, and |
| Abstract: | Objective: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture of membranes (pPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). Methods: One-hundred thirty-eight women with singleton pregnancies complicated by pPROM were included in this study. Amniotic fluid was collected by transabdominal amniocentesis at the time of admission and amniotic fluid concentrations of TAC, FRAP and TBARS were measured. Result: The presence of MIAC and/or HCA did not show any significant differences in the amniotic fluid TAC, FRAP and TBARS concentrations. Positive correlations between gestational age at sampling and amniotic fluid TAC and FRAP concentrations were found (TAC: rho?=?0.32; p?=?0.0002; FRAP: rho?=?0.36; p?p?=?0.004). Conclusions: Oxidative stress is associated with pPROM as indicated by the presence of markers tested in the amniotic fluid; however, oxidative stress markers tested are not influenced by the presence of MIAC or HCA. |
| Keywords: | Infection inflammation preterm delivery |