| CD40-CD40配体对大鼠主动脉平滑肌细胞增殖及迁移影响 |
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| 引用本文： | 严金川,丁澍,梁仪,刘培晶,任国庆,朱建,马根山.CD40-CD40配体对大鼠主动脉平滑肌细胞增殖及迁移影响[J].临床心血管病杂志,2007,23(11):854-857. |
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| 作者姓名： | 严金川 丁澍 梁仪 刘培晶 任国庆 朱建 马根山 |
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| 作者单位： | 1. 江苏大学附属医院心内科,江苏镇江,212001
2. 东南大学附属中大医院心内科 |
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| 基金项目： | 江苏省自然基金资助(No:BK2005069) |
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| 摘 要： | 目的:观察CD40-CD40配体(CD40-CD40L)相互作用对体外培养的大鼠主动脉平滑肌细胞(VSMC)增殖及迁移的影响。方法:应用组织贴块法进行SD大鼠主动脉VSMC原代培养,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法分别测定VSMC增殖,VSMC的迁移采用琼脂糖凝胶刮取法,用倒置显微镜观察。结果:CD40-CD40L相互作用能明显促进VSMC3H-TdR、3H-Leu向细胞的掺入率,两者具有时间、剂量依赖性,CD40-CD40L相互作用随CD40L浓度的增加及刺激时间延长(30h内),能明显增加VSMC迁移率,抗-CD40单克隆抗体能明显抑制上述效应。结论:CD40-CD40L相互作用能明显促进主动脉VSMC增殖和迁移。
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| 关 键 词： | 抗原 CD40 CD40配体 肌 平滑 血管 增殖 |
| 文章编号： | 1001-1439(2007)11-0854-04 |
| 修稿时间： | 2007年3月12日 |
Effect of CD40-CD40 ligand interaction on the proliferation and migration in cultured cells of rat aortic smooth muscle |
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| YAN Jinchuan,DING Shu,LIANG Yi,LIU Peijin,REN Guoqing,ZHU Jian,MA Genshan.Effect of CD40-CD40 ligand interaction on the proliferation and migration in cultured cells of rat aortic smooth muscle[J].Journal of Clinical Cardiology,2007,23(11):854-857. |
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| Authors: | YAN Jinchuan DING Shu LIANG Yi LIU Peijin REN Guoqing ZHU Jian MA Genshan |
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| Institution: | YAN Jinchuan1 DING Shu1 LIANG Yi1 LIU Peijin1REN Guoqing1 ZHU Jian2 MA Genshan2 |
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| Abstract: | Objective:To investigate the effect of CD40-CD40 ligand interaction on the proliferation and migration in cultured aortic vessel smooth muscle cells(VSMC)of rat.Method:The primary passage smooth muscle cells of rats were cultured by tissue mince-sticking method and the 3rd and 4th passage cells were incubated with indicated concentrations of CD40 ligand(CD40L)for various periods.The incorporation of 3H-TdR and 3H-Leu analysis was used to evaluate the cell proliferation.Cell migration was obtained by monolayer cell scrape and observed by reverse microscope.Result:The incorporation of 3H-TdR,3H-Leu and the migration of smooth muscle cells were significantly increased by CD40L with a dose-and time-dependent manner.Anti-CD40 monoclonal antibody significantly suppressed CD40L-induced incorporation of 3H-TdR,3H-Leu and the migration of VSMC.Conclusion:The interaction of CD40-CD40L could promote the proliferation and migration of cultured aortic VSMC of rat. |
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| Keywords: | Antigens CD40 CD40 ligand Muscle smooth vascular Proliferation |
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