| 白藜芦醇抑制人晶状体上皮细胞增殖的信号转导机制 |
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| 引用本文： | 黄露芬,黄秀榕,祁明信,唐宋文. 白藜芦醇抑制人晶状体上皮细胞增殖的信号转导机制[J]. 福建中医学院学报, 2009, 19(4): 50-52 |
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| 作者姓名： | 黄露芬 黄秀榕 祁明信 唐宋文 |
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| 作者单位： | 1. 福建中医学院中西医结合系,福建,福州,350108
2. 福建中医学院附属第二人民医院,福建,福州,350003 |
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| 基金项目： | 福建省卫生厅重点科研项目 |
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| 摘 要： | 目的 探讨白藜芦醇(Res)对重组人碱性成纤维细胞生长因子(rhbFGF)诱导的人晶状体上皮细胞(HLEC)增殖的抑制作用,并从信号转导方面研究其抑制HLEC增殖的作用机制,为寻求防治后发性白内障的有效药物提供实验依据. 方法 采用rhbFGF诱导体外培养的HLEC增殖,流式细胞术(FCM)检测一定浓度的Res对增殖状态的HLEC增殖细胞核抗原(PCNA)蛋白表达的抑制作用:FCM检测不同的细胞内信号转导阻断剂(钙调素抑制剂W7、蛋白激酶C抑制剂H7)对其抑制作用的影响. 结果 Res对HLEC内PCNA蛋白表达有明显的下调作用(P<0.01);W7、H7均明显上调了HLEC内PcNA蛋白表达(P<0.01). 结论 Res有抑制HLEC增殖的作用,其机制可能是通过CaM、PKC等来介导的.
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| 关 键 词： | 白藜芦醇 人晶状体上皮细胞 细胞增殖 抑制 信号转导 |
Signal Transduction Mechanism of Resveratrol in Inhibiting Proliferation of Human Lens Epithelial Cell Induced by Recombinant Human Basic Fibroblast Growth Factor |
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| HUANG Lu-fen,HUANG Xiu-rong,QI Ming-xin,et al. Signal Transduction Mechanism of Resveratrol in Inhibiting Proliferation of Human Lens Epithelial Cell Induced by Recombinant Human Basic Fibroblast Growth Factor[J]. Journal of Fujian College of Traditional Chinese Medicine, 2009, 19(4): 50-52 |
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| Authors: | HUANG Lu-fen HUANG Xiu-rong QI Ming-xin et al |
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| Affiliation: | 1.Department of Integrative Medicine;FUTCM;Fuzhou;Fujian 350108;China;2.The Second Affiliated Hospital;Fujian 350003;China |
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| Abstract: | Objective To investigate the proliferative inhibition of human lens epithelial cell(HLEC) induced by recombinant human basic fibroblast growth factor(rhbFGF) by resveratrol(Res) and further study the mechanism of signal transduction in order to provide scientific basis for pursuing safe and effective natural drugs to prevent and cure after cataract.Methods The proliferation of HLEC cultured in vitro were induced by rhbFGF,the inhibition of a certain concentration of Res on the expression of proliferat-ing c... |
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| Keywords: | resveratrol human lens epithelial cell cell proliferation inhibition signal transduction |
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