| 单双标记技术对T细胞亚群测定的影响 |
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| 引用本文： | 张士发,许静,梁再赋,王良明,冯永山,顾绍裘.单双标记技术对T细胞亚群测定的影响[J].中国皮肤性病学杂志,2000,14(4):273-274. |
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| 作者姓名： | 张士发 许静 梁再赋 王良明 冯永山 顾绍裘 |
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| 作者单位： | 1. 沈阳军区总医院皮肤科辽宁沈阳 110015
2. 中国医科大学第一医院皮肤科 |
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| 摘 要： | 目的 探讨单、双标记技术对Ｔ细胞亚群测定的影响。方法 采用荧光抗体双标记、流式细胞仪对正常４人外周血细胞进行测定。结果 ＣＤ３＾＋ＣＤ４＾＋与ＣＤ４＾＋细胞比较，差异无显著性意义，ＣＤ３＾＋ＣＤ８＾＋与ＣＤ８＾＋比较，有显著性差异（Ｐ〈０．０５），ＣＤ３＾＋ＣＤ４＾＋／ＣＤ３＾＋ＣＤ８＾＋与ＣＤ８＾＋比较，差异有显著性意义（Ｐ〈０．０５）。结论 检测Ｔ细胞亚群应采用双标记技术。
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| 关 键 词： | T细胞亚群 流式细胞仪 测定 |
| 文章编号： | 1001-7089(2000)04-0273-02 |
| 修稿时间： | 1998年12月28 |
Influence of Single and Double Labelling Technique on T Lymphocyte Subsets Determination |
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| ZHANG Shi fa,XU Jing,LIANG Zai fu,et al.Influence of Single and Double Labelling Technique on T Lymphocyte Subsets Determination[J].The Chinese Journal of Dermatovenereology,2000,14(4):273-274. |
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| Authors: | ZHANG Shi fa XU Jing LIANG Zai fu |
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| Institution: | ZHANG Shi fa,XU Jing,LIANG Zai fu,et al Department of Dermatology,General Hospital of Shenyang Millitary Region,Shenyang 110015,China |
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| Abstract: | Objective To Study the influence of single and double labelling technique on T lymphocyte subsets determination.Methods The normal human peripheral blood cell was determined by double antibody which labelled with fluorescein and flowcytometry.Results There was no significant difference between CD 3 CD 4 and CD 4 ,there was significant difference between CD 3 CD 8 and CD 8 ( P <0.05),and between CD 3 CD 4 /CD 3 CD 8 and CD 4 /CD 8 ( P <0.05).Conclusion It is better to use the double labelling technique to determine the T lymphocyte subsets. |
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| Keywords: | T lymphocyte subsets Flowcytometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
