抗肿瘤相关糖蛋白-72单抗在人乳腺癌中的表达

目的 检测肿瘤相关糖蛋白-72（TAG-72）在人乳腺癌细胞系的表达，为利用抗TAG-72单链抗体（single chain variable fragment，scFv）诊治乳腺癌提供实验依据。方法 将人乳腺癌细胞系MCF-7和Bcap-37通过细胞培养至对数生长期，并固定于载玻片上。用原核表达的抗TAG-72scFv经免疫细胞化学方法检测TAG-72在乳腺癌细胞中的表达。结果 TAG-72在乳腺癌细胞系MCF-7中表达为阳性，在Bcap-37中表达为阴性，表明TAG-72在部分人乳腺癌细胞中得以表达。结论 TAG-72在人部分乳腺癌中阳性表达，表明TAG-72抗原可作为一个肿瘤标志物在临床中应用，也为抗TAG-72scFv在乳腺癌诊治中应用奠定了实验基础。

Expression of Anti-Tumor-Associated Glycoprotein-72 Single Chain Variable Fragment in Human Breast Cancer Cells

Objective To investigate the expression of tumor associated glycoprotein-72 (TAG-72) in human breast cancer cells. Methods Two breast cancer cell lines MCF-7 and Bcap-37 were cultured and prepared. TAG-72 expressions in MCF-7 and Bcap-37 were detected immunochemically with anti-TAG-72 single chain variable fragment (scFv). Results TAG-72 was positively expressed in MCF-7 cells but negatively expressed in Bcap-37 cells. Conclusion Tumor associated antigen TAG-72 is expressed in certain human breast cancer cells, which indicate TAG-72 may be used as a tumor maker and anti-TAG-72 scFv may play a role in the diagnosis and treatment of breast cancer.