肝脏低温保存再灌注肝细胞凋亡及其与细胞内钙离子浓度的相关性

目的：研究肝移植过程中供肝低温保存－再灌注期间肝实质细胞凋亡及其相关机制。方法：应用细胞凋亡原位末端标记法并结合电镜观察，检测不同低温保存时间（分别为０、３、６和９小时）的４组兔肝脏在低温保存－再灌注过程中肝实质细胞凋亡发生情况。同时，对各组肝脏再灌注前、后肝实质细胞内游离钙离子浓度（〔Ｃａ＾２＋〕ｉ）进行测定。结果：各组肝脏于低温保存末、组织内未见或仅偶见肝实质细胞凋亡现象。而低温保存后的再灌注

Experimental study on hepatic cell apoptosis and its relationship with intracellular [Ca2+] during cryopreservationreperfusion of isolated livers

Objective:To investigate the nature and possible mechanisms of hepatic cell apoptosis during cryopreservationreperfusion in the process of liver transplantation.Methods:Four groups of rabbit livers,which were cryopreserved for 0,3,6,9 hours respectively,were taken into observation.Apoptosis of hepatic parenchymal cells were analyzed using TdTmediated dUTPbiotin nick end labeling and electron microscope.Levels of intracellular [Ca 2 ] of hepatic parenchymal cells were observed at the time points of both prereperfusion and postreperfusion.Results:Apoptotic hepatic parenchymal cells rarely appeared at the endpoint of cryopreservation in each group but they were obviously seen in reperfusion livers subsequent to cryopreservation.The longer the liver cryopreserved,the greater the number of apoptotic cells.In addition,there was significant positive correlation between the number of apoptotic hepatic parenchymal cells and the intracellular [Ca 2 ].Conclusions:Apoptosis of hepatic parenchymal cells exists in the process of donor liver cryopreservationreperfusion and intracellular [Ca 2 ] overloading might play an important role in inducing such apoptosis.