锌-金属硫蛋白对大鼠皮肤烫伤创面的影响

目的研究Ｗｉｓｔａｒ大鼠深Ⅱ度烧伤后皮肤活力的下降与脂质过氧化反应的关系。方法以氧耗量、琥珀酸脱氢酶、Ｓｃｈｉｆ’ｓ碱及创面愈合时间为指标，以金属硫蛋白作为保护剂外用于烫伤创面，分别采用空白对照及５×１０－６ｍｏｌ／Ｌ，１×１０－５ｍｏｌ／Ｌ两种浓度，测定伤后８，２４，４８ｈ各项指标的变化。结果皮肤氧耗量、琥珀酸脱氢酶活性下降，Ｓｃｈｉｆｓ碱含量明显升高。应用金属硫蛋白保护后，皮肤活性（氧耗及琥珀酸脱氢酶含量）均显著高于对照组（Ｐ＜０．０５或Ｐ＜０．０１），同时Ｓｃｈｉｆ’ｓ碱含量下降（Ｐ＜０．０５或Ｐ＜０．０１），创面愈合时间平均提前２天。结论烫伤组织损伤与脂质过氧化反应有一定的关系，而金属硫蛋白有一定保护作用。

Effects of metallothionein(MT) on burned skin of rats

Objective\ To elucidate the relationship between skin viability and lipid peroxidation after deep partial thickness burn injury. Methods\ Deep partial thickness burn was produced. Metallothionin (MT) in different concentrations (0,510-6mol/L,110-5mol/L) was topically applied. Oxygen consumption, succinate dehydrogenase(SDH), Schiff's base contents and healing time of wound were determined in 24 Wistar rats. Specimens were harvested at 8,24, and 48hour postburn. Results\ Oxygen consumption and SDH value decreased and Schiff's base value increased after burn injury. With the application of MT, oxygen consumption and SDH value of burned skin were significantly higher compared with control group, and Schiff's base value was significantly lower (P<0.05 or P<0.01).The mean healing time of burned wound was two days less in two MT protection groups. Conclusion\ MT may be beneficial in improving postburn skin viability by attenuating lipid peroxidation of burned tissue.