| 黄芪、丹参、山药及其复方对高糖所致雪旺细胞凋亡的保护作用 |
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| 引用本文： | 刘笑迎,张云云,张捷青,丁丽玉,孙爱军,刘建缓. 黄芪、丹参、山药及其复方对高糖所致雪旺细胞凋亡的保护作用[J]. 中药药理与临床, 2010, 0(1) |
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| 作者姓名： | 刘笑迎 张云云 张捷青 丁丽玉 孙爱军 刘建缓 |
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| 作者单位： | 上海中医药大学附属岳阳中西医结合医院神经内科;上海市第二人民医院;上海中医药大学药学院;上海中医药大学附属岳阳中西医结合医院药剂科;上海复旦大学附属中山医院心血管病研究所;上海市公共卫生临床中心; |
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| 基金项目： | 上海市自然科学基金资助(03zr14090) |
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| 摘 要： | 目的:观察中药对高葡萄糖条件下与内皮细胞(以下简称EC)共培养的雪旺细胞(以下简称SC)凋亡的干预作用。方法:建立大鼠SC与EC的共培养模型,根据存活率(形态学和MTT)筛选药物最有效作用浓度。将细胞分为正常共培养SC组、高糖共培养SC组、高糖黄芪组、高糖丹参组、高糖山药组、高糖复方组,通过凋亡率(流式细胞仪)和凋亡相关基因Bcl-2和Casepase-3的mRNA(Realtime PCR)和蛋白(Western Blot)的表达观察细胞凋亡。结果:与正常共培养组相比高糖共培养组SC凋亡率明显增高、Bcl-2mRNA和蛋白表达量明显减少,Casepase-3mRNA和蛋白表达量显著增加。加中药干预后SC凋亡率都明显降低,Bcl-2mRNA和蛋白表达量都明显增加,除高糖山药组Casepase-3mRNA减少和高糖丹参组Casepase-3蛋白减少无明显统计学意义,其余各组Casepase-3mRNA和蛋白表达量均显著减少。中药干预组之间比较,高糖复方组作用最显著。中药干预组与正常组相比,SC凋亡率无明显增高,Bcl-2mRNA和蛋白表达量明显减少,Casepase-3mRNA和蛋白表达量显著增加。结论:中药对高糖环境下与内皮细胞共培养的雪旺细胞的凋亡有保护作用,不同中药作用效果不同,复方效果最佳。
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| 关 键 词： | 黄芪 丹参 山药 内皮细胞 雪旺细胞 高葡萄糖 凋亡 |
Effect of astragalus,salvia,yam and its compound on apoptosis of Schwann cells co-cultured with endothelial cells in high glucose |
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| Liu Xiaoying ,,Zhang Yunyun,Zhang Jieqing,Ding Yuli,Sun Aijun,Liu Jianhuan. Effect of astragalus,salvia,yam and its compound on apoptosis of Schwann cells co-cultured with endothelial cells in high glucose[J]. Pharmacology and Clinics of Chinese Materia Medica, 2010, 0(1) |
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| Authors: | Liu Xiaoying Zhang Yunyun Zhang Jieqing Ding Yuli Sun Aijun Liu Jianhuan |
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| Affiliation: | Liu Xiaoying 1,2,Zhang Yunyun1,Zhang Jieqing,Ding Yuli,Sun Aijun,Liu Jianhuan(1 Department of Neurology,Yueyang Hospital,Shanghai University of TCM,Shanghai 200437,2 Shanghai Second People's Hospital,Shanghai 200010) |
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| Abstract: | Objective:To observe the damage situation of the Schwann cells co-cultured with endothelial cells in high glucose and the protective effect of Chinese herbs.Methods:The co-culture model with Schwann cells and endothelial cells in high glucose was set up,the most effective drug concentration were selected according to the survival rate(morphology and MTT),and the cells were divided into normal glucose co-cultured group,high glucose co-cultured group,Astragalus,Salvia,yam and its compound intervention group.T... |
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| Keywords: | Chinese herbs endothelial cells Schwann cells high glucose co-culture apoptosis |
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