溃疡性结肠炎大鼠肠组织中MDA、SOD、损伤指数的动态变化及其相关性研究

目的探讨氧自由基在溃疡性结肠炎的发病机制及肠组织中丙二醛(MDA),超氧化物歧化酶(SOD)在评价炎症严重程度中的作用。方法用三硝基苯磺酸(TNBS)/乙醇制作实验性溃疡性结肠炎的动物模型,检测实验溃疡性结肠炎肠组织中MDA、SOD、损伤指数的动态变化,并作相关性分析。结果肠组织MDA、损伤指数第3天见升高达高峰后渐下降,肠SOD第3天明显降低后渐升高,至第21天上述各指标均恢复正常。肠组织MDA与损伤指数呈正相关(r=0.823,P<0.01),肠组织SOD与损伤指数呈负相关(r=-0.784,P<0.01)。结论在溃疡性结肠炎发病过程中氧自由基参与了其发病过程,且肠粘膜组织中的MDA上升及SOD下降水平同炎症严重程度在时间上是一致的,可作为炎症严重程度的评价指标。

The Dynamic Changes of MDA、SOD Activity,and Ulceration Index and Their Correlation in Colon Mucosa and in UC Rats

Objective To investigate the effects of oxygen radicals on pathological mechanical and roles of MDA,SOD activity in colon mucosa in assessing inflammatory severity in UC.Methods UC animal models was induced by Trinitrophenylmethylnitramine(TNBS). The dynamic changes of MDA,SOD activity and ulceration index in colon mucus were determined.Results MDA activity in colon mucosa and colon mucosal ulceration index were significantly increased on the 3rd day after induced by TNBS then decreased gradually.But SOD activity in colon mucosa were significantly decreased on the 3rd day,then increased gradually.All were normal on the 21th day.And their changes were synchronous.The colon mucosal ulceration index was significant positive correlated with MDA,but was negative correlated with SOD.The correlation coefficient was 0.823,-0.784 respectively,(P<0.01).Conclusion The pathogenesis of UC is involved with oxygen radicals,furthermore,MDA and SOD activity in colon mucosa can be regarded markers of assessing inflammatory severity in UC.