| Roscovitine对C型尼曼-皮克病小鼠的神经保护作用 |
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| 引用本文： | 张旻,卜碧涛,王伟,Inez Vincent. Roscovitine对C型尼曼-皮克病小鼠的神经保护作用[J]. 卒中与神经疾病, 2005, 12(6): 326-328,333 |
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| 作者姓名： | 张旻 卜碧涛 王伟 Inez Vincent |
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| 作者单位： | 1. 430030,武汉,华中科技大学同济医学院附属同济医院神经内科
2. 美国华盛顿大学病理系 |
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| 基金项目： | 中国科学院资助项目，中国科学院资助项目 |
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| 摘 要： | 目的 观察细胞周期依赖性蛋白激酶特异性抑制剂Roscovitine对C型尼曼-皮克病（NPC）小鼠（npc^-/-）的治疗作用。方法 采用72、144、300和600nmol／d的Roscovitine对5周龄的npc^-/-小鼠（n=6～10）进行2周侧脑室灌注，以DMSO灌注的同龄npc^-/-小鼠（n=6～10）为对照；采用免疫组织化学染色、H＆．E染色和小鼠衣架悬挂试验来进行检测。结果 Roscovitine能显著减少球状轴突的数量，延缓浦肯野细胞的死亡并改善npc^-/-小鼠的运动功能。结论 Roscovitine对NPC的神经系统变性有一定的保护作用。
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| 关 键 词： | C型尼曼-皮克病 细胞周期依赖性蛋白激酶 细胞骨架蛋白过度磷酸化 球状轴突 |
| 文章编号： | 1007-0478（2005）06-0326-03 |
| 收稿时间： | 2005-04-14 |
| 修稿时间： | 2005-04-142005-06-21 |
The neuroprotective effects of roscovitine in Niemann-Pick Disease Type C mice |
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| Zhang Min,Bu Bitao,Wang Wei,Inez Vincent. The neuroprotective effects of roscovitine in Niemann-Pick Disease Type C mice[J]. Stroke and Nervous Diseases, 2005, 12(6): 326-328,333 |
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| Authors: | Zhang Min Bu Bitao Wang Wei Inez Vincent |
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| Affiliation: | Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 |
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| Abstract: | Objective To investigate the neuroprotective effects of cyclin dependent kinase activity inhibitor, roscovitine in Niemann-Pick Type C (NPC) mouse model (npc~ -/- ). Methods Different concentrated roscovitine (72, 144, 300 and 600 nmol/day) were infused into 5 week old npc~ -/- mice (n=6~10) intracerebroventricularly for a 2-week period, and DMSO infused, same aged npc~ -/- mice (n=6~10) were used as control. The immunohistochemistry, H&E staining and mouse coat hanger test were adopted in this research. Result Roscovitine significantly reduced the number of spheroids, modulated Purkinje neuron death, and ameliorated motor defects in npc~ -/- mice. Conclusion The study suggested the neuroprotective effects of roscovitine to the neurodegeneration in NPC. |
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| Keywords: | Roscovitine |
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