| 高效液相色谱法测定2种獐牙菜中龙胆苦苷的含量 |
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| 引用本文： | 康军民,谭健兵,李福双,徐康平. 高效液相色谱法测定2种獐牙菜中龙胆苦苷的含量[J]. 中南药学, 2008, 6(2): 174-176 |
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| 作者姓名： | 康军民 谭健兵 李福双 徐康平 |
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| 作者单位： | [1]湖南省湘潭市韶山医院,湖南湘潭411300; [2]中南大学药学院,长沙410013 |
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| 摘 要： | 目的建立测定川东獐牙菜、显脉獐牙菜中龙胆苦苷含量的高效液相色谱方法。方法HYPERSILBDSC18（4.6mm×200mm,5μm）色谱柱,流动相为甲醇-水梯度洗脱（MeOH%：0min,15%;40min,45%）,流速为1.0mL·min^-1,检测波长为276nm,柱温为25℃。结果龙胆苦苷在0.22～6.50μg线性良好,r=0.99996,平均回收率为96.30%,RSD〈3%（n=6）。结论本方法操作简便,分离效果好,灵敏度高,重现性好,为控制川东獐牙菜和显脉獐牙菜质量提供参考。
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| 关 键 词： | 龙胆苦苷 川东獐牙菜 显脉獐牙菜 高效液相色谱法 含量测定 |
| 文章编号： | 1672-2981(2008)02-0174-03 |
| 修稿时间： | 2007-11-14 |
Content determination of gentiopicroside in 2 species of Swertia of genus by HPLC |
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| KANG Jun-min,TAN Jian-bing,LI Fu-shuang,XU Kang-ping. Content determination of gentiopicroside in 2 species of Swertia of genus by HPLC[J]. Central South Pharmacy, 2008, 6(2): 174-176 |
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| Authors: | KANG Jun-min TAN Jian-bing LI Fu-shuang XU Kang-ping |
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| Affiliation: | KANG Jun-min, TAN Jian-bing, LI Fu-shuang, XU Kang-ping ( Shaoshan Hospital of Xiangtan City, Xiangtan Hunan 411300; School of Pharmaceutical Sciences, Central South University, Changsha 410013) |
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| Abstract: | Objective To establish an HPLC method for the determination of gentiopicroside in Swertia davidi Franch. and Swertia nervosa （G. Don） Wall. Methods A column of HYPERSIL BDS Cls （4.6 mm× 200 mm, 5 μm） was used. The mobile phase consisted of methanol and water with a linear gradient elution from 15% to 45% methanol in 40 minutes. The flow rate was 1.0 mL · min ^-, and the detection wavelength was 276 nm. Results The calibration curve of gentiopicroside was in good linearity over 0.22 - 6.50 μg （r= 0. 999 96）. The average recovery was 96.30% , and RSD was less than 3% （n=6）. Conclusion The method is simple, sensitive, accurate, reproducible, and can be used to control the quality of Swertia davidi Franch and Swertia nervosa （G. Don） Wall. |
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| Keywords: | gentiopicroside Swertiadavidi Franch. Swertia nerwsa （G. Don） Wall. HPLC content determination |
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