临床用氧对新生鼠视网膜发育的影响

目的 研究不同临床用氧方式对新生鼠视网膜血管发育的影响以及相应视网膜血管内皮生长因子(VEGF) 的表达以探讨ROP发病机制,为早产儿临床合理用氧提供实验依据.方法 新生7 d Wistar鼠仔150只,按不同吸氧方式称质量随机分为5组.实验组1: FiO2由75%分5 d逐渐降低直至停氧(每天下降幅度约为10%);实验组2:吸入氧浓度( FiO2 )5 d逐渐由30%升高至75%后突然中断吸氧.实验组3:持续吸75%高氧5 d后骤然停氧;实验组4:持续吸75%高氧5 d后分5 d逐渐降低FiO2 直至停氧;对照组:置于同实验条件下的空气中饲养.视网膜铺片(ADP酶染色法)了解视网膜血管形态改变;眼球切片(HE法)观察视网膜血管的增生和发育情况;免疫组化法观察5组标本视网膜各层VEGF的表达,并进行视网膜组织内VEGF的含量,血管内皮细胞核两者之间相关性分析.结果 ①视网膜铺片和眼球切片HE染色显示实验组2、3视网膜存在缺氧并出现异常新生血管生成的类似ROP表现.②免疫组化结果显示缺氧后视网膜组织内VEGF的表达明显增加,主要分布在视网膜节细胞层和内核层的毛细血管内皮细胞细胞质和细胞核中.视网膜新生血管芽细胞核数(以与内界膜相关联的突破内界膜的内皮细胞核数C表示)与视网膜组织VEGF含量呈正相关(r=0.807,P＜0.01).结论 吸入高浓度氧后突然停氧可严重影响新生鼠视网膜血管的发育,产生类似早产儿视网膜病的病变.因此临床上应严密监测用氧情况,采取逐渐降低氧浓度的吸氧方式.

Effects of oxygen inhalation modes on retinal vessels development in neonatal rat

Objective To investigate the effects of different oxygen inhalation modes on retinal vessels development in neonatal rat in order to approach its etiopathogenesis and provide experimental evidence for proper oxygen therapy for premature.Methods Totally 150 postnatal day 7(P7)Wistar rats were randomly assigned into 5 groups according to different oxygen inhalation modes(n=30 in each group).Experimental group 1 were exposed to 75%,60%,50%,40% and 30% oxygen in turn for one day,followed by room air exposure fo...